Two distinct mechanisms cause heterogeneity of 16S rRNA.

Journal of bacteriology ·Vol. 181 ·No. 1 ·1999-01-00 ·Pages 78-82

Ueda K, Seki T, Kudo T, Yoshida T, Kataoka M

Abstract

To investigate the frequency of heterogeneity among the multiple 16S rRNA genes within a single microorganism, we determined directly the 120-bp nucleotide sequences containing the hypervariable alpha region of the 16S rRNA gene from 475 Streptomyces strains. Display of the direct sequencing patterns revealed the existence of 136 heterogeneous loci among a total of 33 strains. The heterogeneous loci were detected only in the stem region designated helix 10. All of the substitutions conserved the relevant secondary structure. The 33 strains were divided into two groups: one group, including 22 strains, had less than two heterogeneous bases; the other group, including 11 strains, had five or more heterogeneous bases. The two groups were different in their combinations of heterogeneous bases. The former mainly contained transitional substitutions, and the latter was mainly composed of transversional substitutions, suggesting that at least two mechanisms, possibly misincorporation during DNA replication and horizontal gene transfer, cause rRNA heterogeneity.

MeSH Terms

Base Composition Base Sequence DNA Primers/genetics DNA Replication/genetics Gene Transfer, Horizontal Genes, Bacterial Genetic Variation Molecular Sequence Data Nucleic Acid Conformation Phylogeny RNA, Bacterial/chemistry,genetics RNA, Ribosomal, 16S/chemistry,genetics Species Specificity Streptomyces/classification,genetics

Chemicals

DNA Primers RNA, Bacterial RNA, Ribosomal, 16S

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Ueda K

International Center for Biotechnology, Osaka University, Yamada-oka, Suita, Osaka 565, Japan.

Seki T

Kudo T

Yoshida T

Kataoka M

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/JB.181.1.78-82.1999

Published

1999-01-00

Pages

78-82

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC103534

Subset

External Links

PubMed source DOI: 10.1128/JB.181.1.78-82.1999 PMC Full Text View journal details

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Two distinct mechanisms cause heterogeneity of 16S rRNA.

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