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PMID: 9891081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a gene that reverses the immortal phenotype of a subset of cells and is a member of a novel family of transcription factor-like genes.

Molecular and cellular biology ·Vol. 19 ·No. 2 ·1999-02-00 ·Pages 1479-85

Bertram MJ, Bérubé NG, Hang-Swanson X, Ran Q, Leung JK, Bryce S, Spurgers K, Bick RJ, Baldini A, Ning Y, Clark LJ, Parkinson EK, Barrett JC, Smith JR, Pereira-Smith OM

Abstract

Based on the dominance of cellular senescence over immortality, immortal human cell lines have been assigned to four complementation groups for indefinite division. Human chromosomes carrying senescence genes have been identified, including chromosome 4. We report the cloning and identification of a gene, mortality factor 4 (MORF 4), which induces a senescent-like phenotype in immortal cell lines assigned to complementation group B with concomitant changes in two markers for senescence. MORF 4 is a member of a novel family of genes with transcription factor-like motifs. We present here the sequences of the seven family members, their chromosomal locations, and a partial characterization of the three members that are expressed. Elucidation of the mechanism of action of these genes should enhance our understanding of growth regulation and cellular aging.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Division/genetics Cell Line Cell Nucleus/metabolism Cellular Senescence/genetics Gene Expression Genetic Complementation Test Humans Molecular Sequence Data Multigene Family Oligonucleotide Probes/genetics Phenotype RNA, Messenger/genetics,metabolism Sequence Homology, Amino Acid Transcription Factors/genetics,metabolism
Chemicals
MORF4 protein, human MORF4L1P1 protein, human MORF4L2 protein, human Oligonucleotide Probes RNA, Messenger Transcription Factors
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Bertram M J
Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, Texas 77030, USA. [email protected]
Bérubé N G
Hang-Swanson X
Ran Q
Leung J K
Bryce S
Spurgers K
Bick R J
Baldini A
Ning Y
Clark L J
Parkinson E K
Barrett J C
Smith J R
Pereira-Smith O M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.19.2.1479
Email
[email protected]
Published
1999-02-00
Pages
1479-85
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC116076
Subset
IM
Grants
NIA NIH HHS · T32AG00183 · United States
NIA NIH HHS · R37AG05333 · United States
NIA NIH HHS · P01AG13663 · United States
Databases
GENBANK
AF100614, AF100615, AF100616, AF100617, AF100618, AF100619, AF100620
External Links
PubMed source DOI: 10.1128/MCB.19.2.1479 PMC Full Text View journal details
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