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PMID: 9892709 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Selectable viruses and altered susceptibility mutants in Arabidopsis thaliana.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 2 ·1999-01-19 ·Pages 772-7

Whitham SA, Yamamoto ML, Carrington JC

Abstract

The genetic basis for susceptibility or nonsusceptibility of plants to viruses is understood poorly. Two selectable tobacco etch virus (TEV) strains were developed for identification of Arabidopsis thaliana mutants with either gain-of-susceptibility or loss-of-susceptibility phenotypes. These strains conferred a conditional-survival phenotype to Arabidopsis based on systemic expression of herbicide resistance or proherbicide sensitivity genes, thereby facilitating mass selections and screens for Arabidopsis mutants that enhance or suppress TEV replication, cell-to-cell movement, or long-distance movement. A multicomponent mechanism that restricts systemic invasion of TEV was identified through isolation of gain-of-susceptibility mutants with alterations at two loci.

MeSH Terms
Arabidopsis/genetics,virology Chromosome Mapping Crosses, Genetic Genes, Plant/genetics Genetic Predisposition to Disease/genetics Herbicides/pharmacology Mutation/genetics Plant Viruses/pathogenicity
Chemicals
Herbicides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Whitham S A
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
Yamamoto M L
Carrington J C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.2.772
Published
1999-01-19
Pages
772-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC15212
Subset
IM
Grants
NIAID NIH HHS · R37 AI043288 · United States
NIAID NIH HHS · R21 AI043288 · United States
NIAID NIH HHS · R01 AI043288 · United States
NIGMS NIH HHS · F32 GM018529 · United States
NIAID NIH HHS · AI43288 · United States
NIGMS NIH HHS · GM18529 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.2.772 PMC Full Text View journal details
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