Home LiteratureArticle Details
PMID: 99141 Published · ppublish English Journal Article

Histone composition of a chromatin fraction containing ribosomal deoxyribonucleic acid isolated from the macronucleus of Tetrahymena pyriformis.

The Biochemical journal ·Vol. 173 ·No. 1 ·1978-07-01 ·Pages 155-64

Jones RW

Abstract

The histone compositions of a chromatin fraction containing ribosomal DNA and of the remaining macronuclear chromatin of Tetrahymena pyriformis was analysed by gel electrophoresis. These chromatin fractions were used as models for transcriptionally active and inactive chromatin respectively. The extent of histone modification, as indicated by the distribution of histone between differently charged subspecies in acid-urea gels, is not grossly different in the two chromatin fractions. However, histone H1 is present, but may be differently modified in the two chromatin fractions. The histone/DNA ratio in ribosomal chromatin, measured after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of samples of chromatin, was found to be the same whether chromatin was extracted from growing or stationary organisms, and to be approx. 40% of this ratio in the remaining macronuclear chromatin. The implications of these results for the possible structure of transcriptionally active chromatin are discussed.

MeSH Terms
Animals Cell Nucleus/analysis Chromatin/analysis DNA/analysis Electrophoresis, Polyacrylamide Gel Histones/analysis Ribosomes/analysis Tetrahymena pyriformis/analysis
Chemicals
Chromatin Histones DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jones R W
References (39)
39 references, click to expand
  1. Analysis of DNA of isolated chromatin subunits.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3978-82 PMID: 1060078
  2. Structure of transcriptionally active chromatin.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4404-8 PMID: 1060119
  3. Chromatin-like organization of the adenovirus chromosome.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):401-4 PMID: 1061143
  4. Activation of chromatin by acetylation of histone side chains.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3937-41 PMID: 1069278
  5. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70 PMID: 1069279
  6. Processing of newly synthesized histone molecules.
    Science. 1975 Oct 10;190(4210):117-28 PMID: 1166303
  7. Physical studies of chromatin. The recombination of histones with DNA.
    Eur J Biochem. 1976 Feb 2;62(1):21-31 PMID: 1248482
  8. Regulation of transcription of genes of ribosomal rna during amphibian oogenesis. A biochemical and morphological study.
    J Cell Biol. 1976 May;69(2):465-89 PMID: 1262400
  9. Genomic transcriptional activity and the structure of chromatin.
    Nature. 1976 Apr 8;260(5551):495-500 PMID: 1264202
  10. ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
    Proc Natl Acad Sci U S A. 1964 May;51:786-94 PMID: 14172992
  11. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  12. Chromatin structure: deduced from a minichromosome.
    Science. 1975 Mar 28;187(4182):1202-3 PMID: 17754289
  13. Phosphorylation of distinct regions of f1 histone. Relationship to the cell cycle.
    J Biol Chem. 1976 Jun 25;251(12):3685-92 PMID: 180006
  14. Spheroid chromatin units (v bodies).
    Science. 1974 Jan 25;183(4122):330-2 PMID: 4128918
  15. Phosphorylation of liver histone following the administration of glucagon and insulin.
    Proc Natl Acad Sci U S A. 1969 Dec;64(4):1276-83 PMID: 4318347
  16. Subunit structure of chromatin.
    Nature. 1974 Sep 20;251(5472):249-51 PMID: 4422492
  17. Partial purification of the template-active fraction of chromatin: a preliminary report.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2193-7 PMID: 4526205
  18. Free ribosomal RNA genes in the macronucleus of Tetrahymena.
    Proc Natl Acad Sci U S A. 1974 Aug;71(8):3078-81 PMID: 4528573
  19. Phosphorylation of very-lysine-rich histone in Physarum polycephalum. Correlation with chromosome condensation.
    Eur J Biochem. 1973 Feb 15;33(1):131-9 PMID: 4570762
  20. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
    Biochem Biophys Res Commun. 1973 May 15;52(2):504-10 PMID: 4711166
  21. Chromatin structure; oligomers of the histones.
    Science. 1974 May 24;184(4139):865-8 PMID: 4825888
  22. Chromatin structure: a repeating unit of histones and DNA.
    Science. 1974 May 24;184(4139):868-71 PMID: 4825889
  23. The effect of trypsin on nuclease-resistant chromatin fragments.
    J Biol Chem. 1974 Jan 10;249(1):152-6 PMID: 4855624
  24. Changing patterns of histone acetylation and RNA synthesis in regeneration of the liver.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1337-44 PMID: 5240033
  25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  26. Synchronization of the cell cycle of Tetrahymena by stravation and refeeding.
    J Protozool. 1970 Aug;17(3):429-31 PMID: 5476015
  27. Histones of polytene and nonpolytene nuclei of Drosophila melanogaster.
    J Biol Chem. 1971 Mar 25;246(6):1841-8 PMID: 5547707
  28. Chemical studies of histone acetylation. The distribution of epsilon-N-acetyllysine in calf thymus histones.
    J Biol Chem. 1968 Dec 25;243(24):6361-6 PMID: 5726891
  29. High resolution acrylamide gel electrophoresis of histones.
    Arch Biochem Biophys. 1969 Mar;130(1):337-46 PMID: 5778650
  30. Studies on histones. 7. Preparative methods for histone fractions from calf thymus.
    Biochem J. 1964 Jul;92(1):55-9 PMID: 5840387
  31. Subunit structure of rDNA-containing chromatin.
    Biochemistry. 1976 Feb 24;15(4):750-5 PMID: 813764
  32. Purification and characterization of the histones associated with the macronucleus of Tetrahymena.
    Biochemistry. 1976 Mar 23;15(6):1249-56 PMID: 814922
  33. Tetrahymena ribosomal RNA gene chromatin is digested by micrococcal nuclease at sites which have the same regular spacing on the DNA as corresponding sites in the bulk nuclear chromatin.
    Nucleic Acids Res. 1976 Feb;3(2):493-505 PMID: 815891
  34. Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes.
    J Mol Biol. 1976 Jun 25;104(2):455-70 PMID: 820864
  35. Structure of nucleosome core particles of chromatin.
    Nature. 1977 Sep 1;269(5623):29-36 PMID: 895884
  36. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  37. Comparative organization of active transcription units in Oncopeltus fasciatus.
    Cell. 1976 Sep;9(1):131-46 PMID: 975237
  38. Chromatin structure as probed by nucleases and proteases: evidence for the central role of histones H3 and H4.
    Cell. 1976 Sep;9(1):179-93 PMID: 987855
  39. Preparation of chromatin containing ribosomal deoxyribonucleic acid from the macronucleus of Tetrahymena pyriformis.
    Biochem J. 1978 Jul 1;173(1):145-53 PMID: 99140
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1730155
Published
1978-07-01
Pages
155-64
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1185758
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1730155 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2