Home LiteratureArticle Details
PMID: 9925638 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Telomeric chromatin modulates replication timing near chromosome ends.

Genes & development ·Vol. 13 ·No. 2 ·1999-01-15 ·Pages 146-51

Stevenson JB, Gottschling DE

Abstract

Saccharomyces cerevisiae telomeric DNA replicates late in S phase, and telomeric genes are transcriptionally silent. Transcriptional repression of telomere-proximal genes results from silent chromatin initiating at the chromosome end, but the relationship between telomeric chromatin and DNA replication is unknown. Mutations in SIR3, a silent chromatin component, cause telomeric DNA on chromosome V to replicate much earlier because of earlier initiation of a nearby replication origin, the Y' ARS. A second telomere-proximal ARS, from an X element, does not act as an origin in a wild-type strain, whereas in a sir3 cell it does. We conclude that telomeric chromatin has a Sir3-dependent inhibitory effect on DNA replication.

MeSH Terms
Chromatin/genetics DNA Replication/genetics Fungal Proteins/genetics,metabolism Mutation Replication Origin/genetics Repressor Proteins/metabolism S Phase Saccharomyces cerevisiae/cytology,genetics,metabolism Silent Information Regulator Proteins, Saccharomyces cerevisiae Telomere/genetics Time Factors Trans-Activators/genetics,metabolism Transcription, Genetic/genetics
Chemicals
Chromatin Fungal Proteins Repressor Proteins SIR3 protein, S cerevisiae Silent Information Regulator Proteins, Saccharomyces cerevisiae Trans-Activators
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stevenson J B
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637 USA.
Gottschling D E
References (28)
28 references, click to expand
  1. A question of time: replication origins of eukaryotic chromosomes.
    Cell. 1992 Oct 30;71(3):363-6 PMID: 1423601
  2. The structure and evolution of subtelomeric Y' repeats in Saccharomyces cerevisiae.
    Genetics. 1992 Jul;131(3):559-74 PMID: 1628806
  3. A position effect on the time of replication origin activation in yeast.
    Cell. 1992 Jan 24;68(2):333-9 PMID: 1733502
  4. Activation of replication origins within yeast chromosomes.
    Annu Rev Cell Biol. 1991;7:375-402 PMID: 1809350
  5. Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae.
    Cell. 1991 Sep 20;66(6):1279-87 PMID: 1913809
  6. A yeast origin of replication is activated late in S phase.
    Cell. 1991 May 3;65(3):507-15 PMID: 2018976
  7. Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.
    Cell. 1990 Nov 16;63(4):751-62 PMID: 2225075
  8. Lack of positional requirements for autonomously replicating sequence elements on artificial yeast chromosomes.
    Proc Natl Acad Sci U S A. 1989 Feb;86(3):973-7 PMID: 2644653
  9. The localization of replication origins on ARS plasmids in S. cerevisiae.
    Cell. 1987 Nov 6;51(3):463-71 PMID: 2822257
  10. Cloning and characterization of four SIR genes of Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Feb;6(2):688-702 PMID: 3023863
  11. Time of replication of yeast centromeres and telomeres.
    Cell. 1988 Aug 12;54(4):505-13 PMID: 3042152
  12. Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae.
    J Cell Biol. 1980 Apr;85(1):108-15 PMID: 6767729
  13. Eucaryotic DNA: organization of the genome for replication.
    Cell. 1978 Oct;15(2):317-25 PMID: 719745
  14. Overcoming telomeric silencing: a trans-activator competes to establish gene expression in a cell cycle-dependent way.
    Genes Dev. 1994 May 15;8(10):1133-46 PMID: 7926719
  15. Analysis of replication origin function on chromosome III of Saccharomyces cerevisiae.
    Cold Spring Harb Symp Quant Biol. 1993;58:415-23 PMID: 7956055
  16. The topography of chromosome replication in yeast.
    Cold Spring Harb Symp Quant Biol. 1993;58:425-34 PMID: 7956056
  17. Replicator dominance in a eukaryotic chromosome.
    EMBO J. 1994 Jul 15;13(14):3395-400 PMID: 8045266
  18. Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and by SIR3 dosage.
    Genes Dev. 1993 Jul;7(7A):1133-45 PMID: 8319906
  19. An alternative pathway for yeast telomere maintenance rescues est1- senescence.
    Cell. 1993 Apr 23;73(2):347-60 PMID: 8477448
  20. Replication initiates at a confined region during DNA amplification in Sciara DNA puff II/9A.
    Genes Dev. 1993 Jun;7(6):1072-84 PMID: 8504930
  21. Analysis of replication intermediates by two-dimensional agarose gel electrophoresis.
    Methods Enzymol. 1995;262:613-27 PMID: 8594382
  22. Multiple determinants controlling activation of yeast replication origins late in S phase.
    Genes Dev. 1996 Jul 1;10(13):1595-607 PMID: 8682291
  23. The chromosome ends of Saccharomyces cerevisiae.
    Yeast. 1995 Dec;11(16):1553-73 PMID: 8720065
  24. Spreading of transcriptional repressor SIR3 from telomeric heterochromatin.
    Nature. 1996 Sep 5;383(6595):92-6 PMID: 8779721
  25. ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae.
    EMBO J. 1996 Dec 2;15(23):6671-9 PMID: 8978693
  26. Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
    Cell. 1997 Oct 3;91(1):59-69 PMID: 9335335
  27. Yeast heterochromatin: regulation of its assembly and inheritance by histones.
    Cell. 1998 May 1;93(3):325-8 PMID: 9590166
  28. New systems for replicating DNA in vitro.
    Curr Opin Cell Biol. 1998 Jun;10(3):304-10 PMID: 9640529
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.2.146
Published
1999-01-15
Pages
146-51
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316395
Subset
IM
Grants
NIGMS NIH HHS · GM43893 · United States
External Links
PubMed source DOI: 10.1101/gad.13.2.146 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2