...NEB) or severe negative energy balance (SNEB). NEB is an imbalance between energy intake and energy requirements for lac...
...NEB) labeled with Cy5- time course with repeats
...NEB, Mississauga, ON) as described previously [54]. cDNA with incorporated 5-(3-aminoallyl)-2’deoxyuridine-5’-triphospha...
...NEB; n = 5) and cows fed ad libitum to maintain positive energy balance (PEB; n = 5). The NEB cows were feed-restricted...
...NEB) status in early lactation. We used Affymetrix GeneChipÒ Bovine Genome Array to investigate the global gene expre...
...NEB) and dNTP mix [6 mM each in TE buffer (10 mM Tris/1 mM EDTA, pH 7.4; Invitrogen)] for 2 h at 37°C. Reactions were te...
...NEB). The digested DNA was used as the template for in vitro transcription with the AmpliScribe T7-Flash transcription k...
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