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PMID: 10022834 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of RBF in the introduction of G1 regulation during Drosophila embryogenesis.

The EMBO journal ·Vol. 18 ·No. 4 ·1999-02-15 ·Pages 916-25

Du W, Dyson N

Abstract

The first appearance of G1 during Drosophila embryogenesis, at cell cycle 17, is accompanied by the down-regulation of E2F-dependent transcription. Mutant alleles of rbf were generated and analyzed to determine the role of RBF in this process. Embryos lacking both maternal and zygotic RBF products show constitutive expression of PCNA and RNR2, two E2F-regulated genes, indicating that RBF is required for their transcriptional repression. Despite the ubiquitous expression of E2F target genes, most epidermal cells enter G1 normally. Rather than pausing in G1 until the appropriate time for cell cycle progression, many of these cells enter an ectopic S-phase. These results indicate that the repression of E2F target genes by RBF is necessary for the maintenance but not the initiation of a G1 phase. The phenotype of RBF-deficient embryos suggests that rbf has a function that is complementary to the roles of dacapo and fizzy-related in the introduction of G1 during Drosophila embryogenesis.

MeSH Terms
Animals Carrier Proteins Cell Cycle/genetics Cell Cycle Proteins Cyclin E Cyclin-Dependent Kinases/metabolism DNA-Binding Proteins Drosophila/embryology Drosophila Proteins E2F Transcription Factors Embryonic Development Eye/embryology,ultrastructure G1 Phase/genetics Gene Deletion Gene Expression Regulation, Developmental/genetics In Situ Hybridization Microscopy, Electron, Scanning Mutation/genetics Phenotype Repressor Proteins/genetics Retinoblastoma Protein Retinoblastoma-Binding Protein 1 Trans-Activators Transcription Factors/deficiency,genetics
Chemicals
Carrier Proteins Cell Cycle Proteins Cyclin E DNA-Binding Proteins Dp transcription factor, Drosophila Drosophila Proteins E2F Transcription Factors Rbf protein, Drosophila Repressor Proteins Retinoblastoma Protein Retinoblastoma-Binding Protein 1 Trans-Activators Transcription Factors Cyclin-Dependent Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Du W
MGH Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA. [email protected]
Dyson N
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46 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.4.916
Email
[email protected]
Published
1999-02-15
Pages
916-25
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171184
Subset
IM
Grants
NIGMS NIH HHS · R01GM53203 · United States
External Links
PubMed source DOI: 10.1093/emboj/18.4.916 PMC Full Text View journal details
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