The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization.

Molecular & general genetics : MGG ·Vol. 164 ·No. 3 ·1978-09-08 ·Pages 249-54

Schmidt T, Egg AH, Kubli E

Abstract

Transfer RNAAsp2delta was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated "in vitro" with Na [125I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAAsp2 to the left arm of the second chromosome in the regions 29 D and E.

MeSH Terms

Animals Asparagine/metabolism Autoradiography Chromatography, Affinity Chromosome Mapping DNA/genetics Drosophila melanogaster/genetics Genes Nucleic Acid Hybridization RNA, Transfer/genetics Salivary Glands/ultrastructure

Chemicals

Asparagine DNA RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Schmidt T

Egg A H

Kubli E

References (30)

30 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00333153

Published

1978-09-08

Pages

249-54

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00333153

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The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization.

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