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PMID: 96430 Published · ppublish English Journal Article

The localization of tRNA4Glu genes from Drosophila melanogaster by "in situ" hybridization.

Nucleic acids research ·Vol. 5 ·No. 5 ·1978-05-00 ·Pages 1465-78

Kubli E, Schmidt T

Abstract

Transfer RNAGlu4 was isolated from Drosophila melanogaster by affinity chromatography. The tRNA was iodinated "in vitro" with Na[25I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAGlu4 to the right arm of the second chromosome and to the left arm of the third chromosome in the regions 52 F, 56 EF and 62 A.

MeSH Terms
Amino Acyl-tRNA Synthetases/metabolism Animals Chromosomes/analysis DNA/analysis,metabolism Drosophila melanogaster/metabolism Glutamates Nucleic Acid Hybridization Oligoribonucleotides/analysis RNA, Transfer/biosynthesis Ribonucleases
Chemicals
Glutamates Oligoribonucleotides DNA RNA, Transfer Ribonucleases Amino Acyl-tRNA Synthetases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kubli E
Schmidt T
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.5.1465
Published
1978-05-00
Pages
1465-78
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342096
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.5.1465 PMC Full Text View journal details
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