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PMID: 10189374 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The MAL proteolipid is necessary for normal apical transport and accurate sorting of the influenza virus hemagglutinin in Madin-Darby canine kidney cells.

The Journal of cell biology ·Vol. 145 ·No. 1 ·1999-04-05 ·Pages 141-51

Puertollano R, Martín-Belmonte F, Millán J, de Marco MC, Albar JP, Kremer L, Alonso MA

Abstract

The MAL (MAL/VIP17) proteolipid is a nonglycosylated integral membrane protein expressed in a restricted pattern of cell types, including T lymphocytes, myelin-forming cells, and polarized epithelial cells. Transport of the influenza virus hemagglutinin (HA) to the apical surface of epithelial Madin-Darby canine kidney (MDCK) cells appears to be mediated by a pathway involving glycolipid- and cholesterol- enriched membranes (GEMs). In MDCK cells, MAL has been proposed previously as being an element of the protein machinery for the GEM-dependent apical transport pathway. Using an antisense oligonucleotide-based strategy and a newly generated monoclonal antibody to canine MAL, herein we have approached the effect of MAL depletion on HA transport in MDCK cells. We have found that MAL depletion diminishes the presence of HA in GEMs, reduces the rate of HA transport to the cell surface, inhibits the delivery of HA to the apical surface, and produces partial missorting of HA to the basolateral membrane. These effects were corrected by ectopic expression of MAL in MDCK cells whose endogenous MAL protein was depleted. Our results indicate that MAL is necessary for both normal apical transport and accurate sorting of HA.

MeSH Terms
Animals Antibodies, Monoclonal/pharmacology Biological Transport/drug effects Cell Polarity Detergents/pharmacology Dogs Epithelial Cells/metabolism Hemagglutinin Glycoproteins, Influenza Virus/metabolism Kidney Membrane Lipids/metabolism Membrane Proteins/metabolism Membrane Transport Proteins Myelin Proteins Myelin and Lymphocyte-Associated Proteolipid Proteins Oligonucleotides, Antisense/pharmacology Proteolipids/antagonists & inhibitors,genetics,immunology,physiology Rats Rats, Wistar Transfection
Chemicals
Antibodies, Monoclonal Detergents Hemagglutinin Glycoproteins, Influenza Virus Mal protein, rat Membrane Lipids Membrane Proteins Membrane Transport Proteins Myelin Proteins Myelin and Lymphocyte-Associated Proteolipid Proteins Oligonucleotides, Antisense Proteolipids hemagglutinin, avian influenza A virus
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Puertollano R
Centro de Biología Molecular "Severo Ochoa," Universidad Autónoma de Madrid and Consejo Superior de Investigaciones Científicas, Cantoblanco, Madrid, Spain.
Martín-Belmonte F
Millán J
de Marco M C
Albar J P
Kremer L
Alonso M A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.145.1.141
Published
1999-04-05
Pages
141-51
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2148211
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.145.1.141 PMC Full Text View journal details
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