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PMID: 10200310 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Escherichia coli genes regulated by cell-to-cell signaling.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 8 ·1999-04-13 ·Pages 4610-4

Baca-DeLancey RR, South MM, Ding X, Rather PN

Abstract

Utilizing the bicistronic reporter transposon mini-Tn5 lacZ-tet/1, we have identified lacZ fusions to four Escherichia coli genes/operons that are strongly activated by the accumulation of self-produced extracellular signals. These fusions were designated cma9, cma48, cma113, and cma114 for conditioned medium activated. Each of the cma fusions was expressed in a growth phase-dependent manner, and the presence of conditioned medium from a stationary phase E. coli culture resulted in the premature activation of these fusions in cells at early to mid-logarithmic phase. The cma48 and cma114 fusions were dependent on RpoS for growth phase expression and response to extracellular factors. The extracellular factors that activated the cma9, cma48, and cma114 fusions were produced in both rich complex and defined minimal media. The cma fusions were shown to be within the cysK (cma9), astD (cma48), tnaB (cma113), and gabT (cma114) genes. These genes function in the uptake, synthesis, or degradation of amino acids that yield pyruvate and succinate.

MeSH Terms
DNA Transposable Elements Escherichia coli/genetics,growth & development,physiology Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Reporter Kinetics Operon Recombinant Fusion Proteins/biosynthesis Signal Transduction beta-Galactosidase/biosynthesis,genetics
Chemicals
DNA Transposable Elements Recombinant Fusion Proteins beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baca-DeLancey R R
Department of Medicine, Case Western Reserve University School of Medicine and Research Service, Department of Veterans Affairs, Cleveland, OH 44106, USA.
South M M
Ding X
Rather P N
References (38)
38 references, click to expand
  1. Control of cell division in Escherichia coli: regulation of transcription of ftsQA involves both rpoS and SdiA-mediated autoinduction.
    Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):336-41 PMID: 8552633
  2. C-factor: a cell-cell signaling protein required for fruiting body morphogenesis of M. xanthus.
    Cell. 1990 Apr 6;61(1):19-26 PMID: 2107980
  3. Biochemical and genetic characterization of a competence pheromone from B. subtilis.
    Cell. 1994 Apr 22;77(2):207-16 PMID: 8168130
  4. Quorum-sensing acts at initiation of chromosomal replication in Escherichia coli.
    Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15694-9 PMID: 9861032
  5. An extracellular factor regulating expression of the chromosomal aminoglycoside 2'-N-acetyltransferase of Providencia stuartii.
    Antimicrob Agents Chemother. 1997 Aug;41(8):1749-54 PMID: 9257754
  6. The complete genome sequence of Escherichia coli K-12.
    Science. 1997 Sep 5;277(5331):1453-62 PMID: 9278503
  7. An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae.
    Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11140-4 PMID: 7479953
  8. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  9. Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication.
    Science. 1993 May 21;260(5111):1127-30 PMID: 8493556
  10. The involvement of cell-to-cell signals in the development of a bacterial biofilm.
    Science. 1998 Apr 10;280(5361):295-8 PMID: 9535661
  11. Intercellular signaling is required for developmental gene expression in Myxococcus xanthus.
    Dev Biol. 1986 Sep;117(1):267-76 PMID: 3017795
  12. Regulation of transcription of katE and katF in Escherichia coli.
    J Bacteriol. 1990 Dec;172(12):6713-20 PMID: 2254248
  13. Nucleotide sequence of the structural gene for tryptophanase of Escherichia coli K-12.
    J Bacteriol. 1981 Sep;147(3):787-96 PMID: 6268608
  14. Branched-chain fatty acids: the case for a novel form of cell-cell signalling during Myxococcus xanthus development.
    Mol Microbiol. 1995 Apr;16(2):171-5 PMID: 7565080
  15. Extracellular complementation of a developmental mutation implicates a small sporulation protein in aerial mycelium formation by S. coelicolor.
    Cell. 1991 May 17;65(4):641-50 PMID: 2032288
  16. Cell-cell communication regulates the effects of protein aspartate phosphatases on the phosphorelay controlling development in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1549-53 PMID: 8643670
  17. DNA sequences of the cysK regions of Salmonella typhimurium and Escherichia coli and linkage of the cysK regions to ptsH.
    J Bacteriol. 1988 Jul;170(7):3150-7 PMID: 3290198
  18. An exported peptide functions intracellularly to contribute to cell density signaling in B. subtilis.
    Cell. 1997 Jun 13;89(6):917-25 PMID: 9200610
  19. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators.
    J Bacteriol. 1994 Jan;176(2):269-75 PMID: 8288518
  20. How and why bacteria talk to each other.
    Cell. 1993 Jun 4;73(5):873-85 PMID: 8500179
  21. Molecular organization of the Escherichia coli gab cluster: nucleotide sequence of the structural genes gabD and gabP and expression of the GABA permease gene.
    Arch Microbiol. 1993;160(6):454-60 PMID: 8297211
  22. Induced cell aggregation and mating in Streptococcus faecalis: evidence for a bacterial sex pheromone.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3479-83 PMID: 98769
  23. A new family of integral membrane proteins involved in transport of aromatic amino acids in Escherichia coli.
    J Bacteriol. 1991 May;173(10):3231-4 PMID: 2022620
  24. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction.
    Nature. 1993 Apr 1;362(6419):448-50 PMID: 8464476
  25. Extracellular control of spore formation in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4369-73 PMID: 3132711
  26. Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli.
    J Bacteriol. 1998 Aug;180(16):4278-86 PMID: 9696779
  27. A kinetic study of the reaction mechanism of tryptophanase-catalyzed reactions.
    J Biol Chem. 1967 Jun 25;242(12):2793-9 PMID: 5338506
  28. Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathway.
    Mol Microbiol. 1994 Jul;13(2):273-86 PMID: 7984107
  29. The bacterial 'enigma': cracking the code of cell-cell communication.
    Mol Microbiol. 1995 May;16(4):615-24 PMID: 7476157
  30. Quorum sensing in Escherichia coli and Salmonella typhimurium.
    Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7046-50 PMID: 9618536
  31. Quorum sensing by peptide pheromones and two-component signal-transduction systems in Gram-positive bacteria.
    Mol Microbiol. 1997 Jun;24(5):895-904 PMID: 9219998
  32. Molecular analysis of two genes of the Escherichia coli gab cluster: nucleotide sequence of the glutamate:succinic semialdehyde transaminase gene (gabT) and characterization of the succinic semialdehyde dehydrogenase gene (gabD).
    J Bacteriol. 1990 Dec;172(12):7035-42 PMID: 2254272
  33. The dps promoter is activated by OxyR during growth and by IHF and sigma S in stationary phase.
    Mol Microbiol. 1994 Jul;13(2):265-72 PMID: 7984106
  34. Sensing starvation: a homoserine lactone--dependent signaling pathway in Escherichia coli.
    Science. 1994 Jul 22;265(5171):537-9 PMID: 7545940
  35. New ways to study developmental genes in spore-forming bacteria.
    Science. 1985 Apr 19;228(4697):285-91 PMID: 17790214
  36. Early and late responses of TOL promoters to pathway inducers: identification of postexponential promoters in Pseudomonas putida with lacZ-tet bicistronic reporters.
    J Bacteriol. 1993 Nov;175(21):6902-7 PMID: 8226632
  37. An extracellular factor regulates expression of sdiA, a transcriptional activator of cell division genes in Escherichia coli.
    J Bacteriol. 1996 May;178(10):2742-8 PMID: 8631660
  38. Cell density control of staphylococcal virulence mediated by an octapeptide pheromone.
    Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12055-9 PMID: 8618843
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.8.4610
Published
1999-04-13
Pages
4610-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC16380
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.96.8.4610 PMC Full Text View journal details
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