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PMID: 10210198 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The influence of C-terminal extension on the structure of the "J-domain" in E. coli DnaJ.

Protein science : a publication of the Protein Society ·Vol. 8 ·No. 1 ·1999-01-00 ·Pages 203-14

Huang K, Flanagan JM, Prestegard JH

Abstract

Two different recombinant constructs of the N-terminal domain in Escherichia coli DnaJ were uniformly labeled with nitrogen-15 and carbon-13. One, DnaJ(1-78), contains the complete "J-domain," and the other, DnaJ(1-104), contains both the "J-domain" and a conserved "G/F" extension at the C-terminus. The three-dimensional structures of these proteins have been determined by heteronuclear NMR experiments. In both proteins the "J-domain" adopts a compact structure consisting of a helix-turn-helix-loop-helix-turn-helix motif. In contrast, the "G/F" region in DnaJ(1-104) does not fold into a well-defined structure. Nevertheless, the "G/F" region has been found to have an effect on the packing of the helices in the "J-domain" in DnaJ(1-104). Particularly, the interhelical angles between Helix IV and other helices are significantly different in the two structures. In addition, there are some local conformational changes in the loop region connecting the two central helices. These structural differences in the "J-domain" in the presence of the "G/F" region may be related to the observation that DnaJ (1-78) is incapable of stimulating the ATPase activity of the molecular chaperone protein DnaK despite evidence that sites mediating the binding of DnaJ to DnaK are located in the 1-78 segment.

MeSH Terms
Amino Acid Sequence Escherichia coli/chemistry Escherichia coli Proteins HSP40 Heat-Shock Proteins Heat-Shock Proteins/chemistry Helix-Loop-Helix Motifs Helix-Turn-Helix Motifs Magnetic Resonance Spectroscopy Molecular Sequence Data Protein Conformation Recombinant Proteins/chemistry
Chemicals
DnaJ protein, E coli Escherichia coli Proteins HSP40 Heat-Shock Proteins Heat-Shock Proteins Recombinant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Huang K
Department of Chemistry, Yale University, New Haven, Connecticut 06520-8107, USA.
Flanagan J M
Prestegard J H
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Article Info
Journal
Protein science : a publication of the Protein Society
Abbr.
Protein Sci
ISSN
0961-8368
DOI
10.1110/ps.8.1.203
Published
1999-01-00
Pages
203-14
Language
English
Region
United States
NLM ID
9211750
PMCID
PMC2144109
Subset
IM
Grants
NIGMS NIH HHS · GM 53181 · United States
External Links
PubMed source DOI: 10.1110/ps.8.1.203 PMC Full Text View journal details
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