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PMID: 10225911 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vivo analysis of secreted aspartyl proteinase expression in human oral candidiasis.

Infection and immunity ·Vol. 67 ·No. 5 ·1999-05-00 ·Pages 2482-90

Naglik JR, Newport G, White TC, Fernandes-Naglik LL, Greenspan JS, Greenspan D, Sweet SP, Challacombe SJ, Agabian N

Abstract

Secreted aspartyl proteinases are putative virulence factors in Candida infections. Candida albicans possesses at least nine members of a SAP gene family, all of which have been sequenced. Although the expression of the SAP genes has been extensively characterized under laboratory growth conditions, no studies have analyzed in detail the in vivo expression of these proteinases in human oral colonization and infection. We have developed a reliable and sensitive procedure to detect C. albicans mRNA from whole saliva of patients with oral C. albicans infection and those with asymptomatic Candida carriage. The reverse transcription-PCR protocol was used to determine which of the SAP1 to SAP7 genes are expressed by C. albicans during colonization and infection of the oral cavity. SAP2 and the SAP4 to SAP6 subfamily were the predominant proteinase genes expressed in the oral cavities of both Candida carriers and patients with oral candidiasis; SAP4, SAP5, or SAP6 mRNA was detected in all subjects. SAP1 and SAP3 transcripts were observed only in patients with oral candidiasis. SAP7 mRNA expression, which has never been demonstrated under laboratory conditions, was detected in several of the patient samples. All seven SAP genes were simultaneously expressed in some patients with oral candidiasis. This is the first detailed study showing that the SAP gene family is expressed by C. albicans during colonization and infection in humans and that C. albicans infection is associated with the differential expression of individual SAP genes which may be involved in the pathogenesis of oral candidiasis.

MeSH Terms
AIDS-Related Opportunistic Infections/etiology,microbiology Adult Aspartic Acid Endopeptidases/genetics,metabolism Base Sequence Candida albicans/enzymology,genetics,pathogenicity Candidiasis, Oral/etiology,microbiology Case-Control Studies DNA Primers/genetics Female Gene Expression Genes, Fungal Humans Male Middle Aged Multigene Family RNA, Fungal/genetics,metabolism RNA, Messenger/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction/statistics & numerical data Sensitivity and Specificity Virulence/genetics,physiology
Chemicals
DNA Primers RNA, Fungal RNA, Messenger Aspartic Acid Endopeptidases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Naglik J R
Oral AIDS Research Unit, Department of Oral Medicine and Pathology, GKT Guy's Hospital, London SE1 9RT, United Kingdom.
Newport G
White T C
Fernandes-Naglik L L
Greenspan J S
Greenspan D
Sweet S P
Challacombe S J
Agabian N
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.67.5.2482-2490.1999
Published
1999-05-00
Pages
2482-90
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC115994
Subset
IM
Grants
NIDCR NIH HHS · P01 DE007946 · United States
NIAID NIH HHS · AI33317 · United States
NIDCR NIH HHS · P01-DE-07946 · United States
External Links
PubMed source DOI: 10.1128/IAI.67.5.2482-2490.1999 PMC Full Text View journal details
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