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PMID: 10359606 Published · ppublish English Journal Article

Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex.

Molecular biology of the cell ·Vol. 10 ·No. 6 ·1999-06-00 ·Pages 1923-38

Marzioch M, Henthorn DC, Herrmann JM, Wilson R, Thomas DY, Bergeron JJ, Solari RC, Rowley A

Abstract

Six new members of the yeast p24 family have been identified and characterized. These six genes, named ERP1-ERP6 (for Emp24p- and Erv25p-related proteins) are not essential, but deletion of ERP1 or ERP2 causes defects in the transport of Gas1p, in the retention of BiP, and deletion of ERP1 results in the suppression of a temperature-sensitive mutation in SEC13 encoding a COPII vesicle coat protein. These phenotypes are similar to those caused by deletion of EMP24 or ERV25, two previously identified genes that encode related p24 proteins. Genetic and biochemical studies demonstrate that Erp1p and Erp2p function in a heteromeric complex with Emp24p and Erv25p.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport Carrier Proteins/genetics,metabolism Fungal Proteins/genetics,metabolism Gene Deletion Genes, Fungal HSP70 Heat-Shock Proteins/genetics,metabolism Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutation Phenotype Saccharomyces cerevisiae Proteins Vesicular Transport Proteins Yeasts/genetics,metabolism
Chemicals
Carrier Proteins EMP24 protein, S cerevisiae ERV25 protein, S cerevisiae Fungal Proteins HSP70 Heat-Shock Proteins KAR2 protein, yeast Membrane Proteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Marzioch M
Cell Biology Unit, Glaxo-Wellcome Research and Development, Stevenage, Hertfordshire, SG1 2NY, United Kingdom.
Henthorn D C
Herrmann J M
Wilson R
Thomas D Y
Bergeron J J
Solari R C
Rowley A
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.10.6.1923
Published
1999-06-00
Pages
1923-38
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25390
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.10.6.1923 PMC Full Text View journal details
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