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PMID: 10364158 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cell cycle-regulated histone acetylation required for expression of the yeast HO gene.

Genes & development ·Vol. 13 ·No. 11 ·1999-06-01 ·Pages 1412-21

Krebs JE, Kuo MH, Allis CD, Peterson CL

Abstract

Expression of the yeast HO gene in late G1 of the cell cycle requires the SWI/SNF chromatin remodeling complex, the Gcn5p histone acetyltransferase, and two different sequence-specific transcriptional activators, Swi5p and Swi4p/Swi6p. We have used chromatin immunoprecipitation assays to investigate the role of each of these trans-acting factors in establishing a cell cycle-regulated domain of histone acetylation surrounding the HO upstream regulatory region. We detect a approximately 1-kb domain of H3 and H4 acetylation that is established in mid-G1, prior to and independent of HO transcription, which then declines with kinetics similar to inactivation of HO. This cell cycle burst of histone acetylation requires Gcn5p, SWI/SNF, and the Swi5p activator, but occurs in the absence of the Swi4p activator. We also find that inactivation of the Sin3p/Rpd3p deacetylase complex leads to a high level of acetylation at the HO locus throughout the cell cycle. We propose a sequential model for activation of HO in which the Swi5p-dependent recruitment of the Gcn5p acetyltransferase requires chromatin remodeling events by the SWI/SNF complex.

MeSH Terms
Acetylation Cell Cycle Cell Cycle Proteins DNA-Binding Proteins Deoxyribonucleases, Type II Site-Specific/genetics Fungal Proteins/metabolism G1 Phase Gene Expression Regulation, Fungal Genes, Fungal Histone Deacetylases/metabolism Histones/metabolism Nucleosomes Promoter Regions, Genetic Protein Kinases/metabolism Repressor Proteins S Phase Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Transcription Factors/metabolism
Chemicals
Cell Cycle Proteins DNA-Binding Proteins Fungal Proteins Histones Nucleosomes Repressor Proteins SIN3 protein, S cerevisiae SWI5 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Protein Kinases HO protein, S cerevisiae SCEI protein, S cerevisiae Deoxyribonucleases, Type II Site-Specific RPD3 protein, S cerevisiae Histone Deacetylases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Krebs J E
Program in Molecular Medicine and Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA.
Kuo M H
Allis C D
Peterson C L
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.11.1412
Published
1999-06-01
Pages
1412-21
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316758
Subset
IM
Grants
NIGMS NIH HHS · R01 GM049650 · United States
NIGMS NIH HHS · R37 GM049650 · United States
NIGMS NIH HHS · GM49650 · United States
External Links
PubMed source DOI: 10.1101/gad.13.11.1412 PMC Full Text View journal details
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