Home LiteratureArticle Details
PMID: 10383955 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the major superoxide dismutase of Staphylococcus aureus and its role in starvation survival, stress resistance, and pathogenicity.

Journal of bacteriology ·Vol. 181 ·No. 13 ·1999-07-00 ·Pages 3898-903

Clements MO, Watson SP, Foster SJ

Abstract

A Staphylococcus aureus mutant (SPW1) which is unable to survive long-term starvation was shown to have a transposon insertion within a gene homologous to the sodA family of manganese-dependent superoxide dismutases (SOD). Whole-cell lysates of the parental 8325-4 strain demonstrated three zones of SOD activity by nondenaturing gel electrophoresis. The activities of two of these zones were dependent on manganese for activity and were absent in SPW1. The levels of SOD activity and sodA expression were growth-phase dependent, occurring most during postexponential phase. This response was also dependent on the level of aeration of the culture, with highest activity and expression occurring only under high aeration. Expression of sodA and, consequently, SOD activity could be induced by methyl viologen but only during the transition from exponential- to postexponential-phase growth. SPW1 was less able to survive amino acid limitation and acid stress but showed no alteration in pathogenicity in a mouse abscess model of infection compared to the parental strain 8325-4.

MeSH Terms
Acids Adaptation, Biological Amino Acid Sequence Animals Bacterial Proteins/genetics,metabolism Cloning, Molecular Enzyme Induction Manganese Mice Molecular Sequence Data Mutagenesis, Insertional Oxidative Stress Sequence Analysis, DNA Sequence Homology, Amino Acid Staphylococcus aureus/enzymology,pathogenicity,physiology Superoxide Dismutase/genetics,metabolism
Chemicals
Acids Bacterial Proteins Manganese SodA protein, Bacteria Superoxide Dismutase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clements M O
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom.
Watson S P
Foster S J
References (45)
45 references, click to expand
  1. A superoxide dismutase mimic protects sodA sodB Escherichia coli against aerobic heating and stationary-phase death.
    Arch Biochem Biophys. 1995 Sep 10;322(1):291-4 PMID: 7574689
  2. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus.
    Virology. 1967 Sep;33(1):155-66 PMID: 4227577
  3. Molecular characterization and functional analysis of the major autolysin of Staphylococcus aureus 8325/4.
    J Bacteriol. 1995 Oct;177(19):5723-5 PMID: 7559367
  4. Characterization of superoxide dismutase genes from gram-positive bacteria by polymerase chain reaction using degenerate primers.
    FEMS Microbiol Lett. 1995 Aug 15;131(1):41-5 PMID: 7557308
  5. Cloning and analysis of sodC, encoding the copper-zinc superoxide dismutase of Escherichia coli.
    J Bacteriol. 1996 May;178(9):2564-71 PMID: 8626323
  6. Periplasmic copper-zinc superoxide dismutase of Legionella pneumophila: role in stationary-phase survival.
    J Bacteriol. 1996 Mar;178(6):1578-84 PMID: 8626284
  7. Involvement of superoxide and myeloperoxidase in oxygen-dependent killing of Staphylococcus aureus by neutrophils.
    Infect Immun. 1996 Sep;64(9):3512-7 PMID: 8751892
  8. Sigma-B, a putative operon encoding alternate sigma factor of Staphylococcus aureus RNA polymerase: molecular cloning and DNA sequencing.
    J Bacteriol. 1996 Oct;178(20):6036-42 PMID: 8830703
  9. Role of bacterial Mn-cofactored superoxide dismutase in oxidative stress responses, nasopharyngeal colonization, and sustained bacteremia caused by Haemophilus influenzae type b.
    Infect Immun. 1997 Jul;65(7):2700-6 PMID: 9199439
  10. Bacterial copper- and zinc-cofactored superoxide dismutase contributes to the pathogenesis of systemic salmonellosis.
    Mol Microbiol. 1997 Aug;25(4):785-96 PMID: 9379906
  11. Periplasmic superoxide dismutase in meningococcal pathogenicity.
    Infect Immun. 1998 Jan;66(1):213-7 PMID: 9423860
  12. Transcriptional and post-transcriptional regulation by nickel of sodN gene encoding nickel-containing superoxide dismutase from Streptomyces coelicolor Müller.
    Mol Microbiol. 1998 Jan;27(1):187-95 PMID: 9466266
  13. Overexpression of a hydrogen peroxide-resistant periplasmic Cu,Zn superoxide dismutase protects Escherichia coli from macrophage killing.
    Biochem Biophys Res Commun. 1998 Feb 24;243(3):804-7 PMID: 9501009
  14. Characterization of the starvation-survival response of Staphylococcus aureus.
    J Bacteriol. 1998 Apr;180(7):1750-8 PMID: 9537371
  15. Molecular cloning and nucleotide sequence of the superoxide dismutase gene and characterization of its product from Bacillus subtilis.
    J Bacteriol. 1998 Jul;180(14):3697-703 PMID: 9658017
  16. Maintenance energy requirement: what is required for stasis survival of Escherichia coli?
    Biochim Biophys Acta. 1998 Jun 10;1365(1-2):225-31 PMID: 9693738
  17. Starvation recovery of Staphylococcus aureus 8325-4.
    Microbiology. 1998 Jul;144 ( Pt 7):1755-63 PMID: 9695909
  18. The role of environmental factors in the regulation of virulence-determinant expression in Staphylococcus aureus 8325-4.
    Microbiology. 1998 Sep;144 ( Pt 9):2469-79 PMID: 9782494
  19. The Staphylococcus aureus alternative sigma factor sigmaB controls the environmental stress response but not starvation survival or pathogenicity in a mouse abscess model.
    J Bacteriol. 1998 Dec;180(23):6082-9 PMID: 9829915
  20. Isolation and characterization of Staphylococcus aureus starvation-induced, stationary-phase mutants defective in survival or recovery.
    Microbiology. 1998 Nov;144 ( Pt 11):3159-69 PMID: 9846752
  21. CtaA of Staphylococcus aureus is required for starvation survival, recovery, and cytochrome biosynthesis.
    J Bacteriol. 1999 Jan;181(2):501-7 PMID: 9882664
  22. Distinguishing between Mn-containing and Fe-containing superoxide dismutases in crude extracts of cells.
    Arch Biochem Biophys. 1980 May;201(2):551-5 PMID: 6994652
  23. A hybrid superoxide dismutase containing both functional iron and manganese.
    J Biol Chem. 1984 May 10;259(9):5932-6 PMID: 6371011
  24. The role of oxygen and its derivatives in microbial pathogenesis and host defense.
    Annu Rev Microbiol. 1984;38:27-48 PMID: 6388493
  25. Iron- and manganese-containing superoxide dismutases can be distinguished by analysis of their primary structures.
    FEBS Lett. 1988 Mar 14;229(2):377-82 PMID: 3345848
  26. Transcriptional and posttranscriptional regulation of manganese superoxide dismutase biosynthesis in Escherichia coli, studied with operon and protein fusions.
    J Bacteriol. 1988 Jun;170(6):2511-20 PMID: 3131302
  27. DNA damage and oxygen radical toxicity.
    Science. 1988 Jun 3;240(4857):1302-9 PMID: 3287616
  28. Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus.
    J Bacteriol. 1988 Sep;170(9):4365-72 PMID: 2457579
  29. The electron transport chain of the microbicidal oxidase of phagocytic cells and its involvement in the molecular pathology of chronic granulomatous disease.
    J Clin Invest. 1989 Jun;83(6):1785-93 PMID: 2656760
  30. A chemically defined medium for slime production by coagulase-negative staphylococci.
    J Med Microbiol. 1991 Mar;34(3):143-7 PMID: 2010904
  31. Genetic systems in staphylococci.
    Methods Enzymol. 1991;204:587-636 PMID: 1658572
  32. Regulation of bacterial oxidative stress genes.
    Annu Rev Genet. 1991;25:315-37 PMID: 1667460
  33. Copper-zinc superoxide dismutase in Haemophilus species.
    J Gen Microbiol. 1992 Mar;138(3):517-22 PMID: 1593263
  34. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  35. Quantitative aspects of the production of superoxide anion radical by milk xanthine oxidase.
    J Biol Chem. 1970 Aug 25;245(16):4053-7 PMID: 5496991
  36. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.
    Anal Biochem. 1971 Nov;44(1):276-87 PMID: 4943714
  37. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  38. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase.
    Eur J Biochem. 1974 Sep 16;47(3):469-74 PMID: 4215654
  39. The biology of oxygen radicals.
    Science. 1978 Sep 8;201(4359):875-80 PMID: 210504
  40. Superoxide radical and the oxygen enhancement of the toxicity of paraquat in Escherichia coli.
    J Biol Chem. 1978 Nov 25;253(22):8143-8 PMID: 213429
  41. Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr.
    Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6462-6 PMID: 1321441
  42. Functional differences between manganese and iron superoxide dismutases in Escherichia coli K-12.
    J Biol Chem. 1992 Dec 5;267(34):24253-8 PMID: 1447175
  43. Expression of superoxide dismutase in Listeria monocytogenes.
    Appl Environ Microbiol. 1994 Jul;60(7):2360-6 PMID: 8074517
  44. Escherichia coli expresses a copper- and zinc-containing superoxide dismutase.
    J Biol Chem. 1994 Oct 14;269(41):25310-4 PMID: 7929223
  45. Superoxide radical and superoxide dismutases.
    Annu Rev Biochem. 1995;64:97-112 PMID: 7574505
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.13.3898-3903.1999
Published
1999-07-00
Pages
3898-903
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93877
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.181.13.3898-3903.1999 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2