Home LiteratureArticle Details
PMID: 10400808 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mass determination of rous sarcoma virus virions by scanning transmission electron microscopy.

Journal of virology ·Vol. 73 ·No. 8 ·1999-08-00 ·Pages 7050-5

Vogt VM, Simon MN

Abstract

The internal structural protein of retroviruses, Gag, comprises most of the mass of the virion, and Gag itself can give rise to virus-like particles when expressed in appropriate cells. Previously the stoichiometry of Gag in virions was inferred from indirect measurements carried out 2 decades ago. We now have directly determined the masses of individual particles of the prototypic avian retrovirus, Rous sarcoma virus (RSV), by using scanning transmission electron microscopy. In this technique, the number of scattered electrons in the dark-field image integrated over an individual freeze-dried virus particle on a grid is directly proportional to its mass. The RSV virions had a mean mass of 2.5 x 10(8) Da, corresponding to about 1,500 Gag molecules per virion. The population of virions was not homogeneous, with about one-third to two-thirds of the virions deviating from the mean by more than 10% of the mass in two respective preparations. The mean masses for virions carrying genomes of 7.4 or 9.3 kb were indistinguishable, suggesting that mass variability is not due to differences in RNA incorporation.

MeSH Terms
Animals Avian Sarcoma Viruses/ultrastructure Microscopy, Electron, Scanning Virion/ultrastructure
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vogt V M
Section of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA. [email protected]
Simon M N
References (40)
40 references, click to expand
  1. Tumor virus RNA's.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):825-34 PMID: 4293266
  2. Purification and properties of a fifth major viral gag protein from avian sarcoma and leukemia viruses.
    J Virol. 1983 Feb;45(2):648-58 PMID: 6300427
  3. Studies on the RNA from avian myeloblastosis virus.
    Virology. 1969 Jan;37(1):124-31 PMID: 4302800
  4. Glycoprotein components of avian and murine RNA tumor viruses.
    Virology. 1970 Aug;41(4):631-46 PMID: 4319782
  5. Phospholipid composition of Rous sarcoma virus, host cell membranes and other enveloped RNA viruses.
    Virology. 1971 Oct;46(1):106-16 PMID: 4108030
  6. Identification of the spike proteins of Rous sarcoma virus.
    Virology. 1971 Nov;46(2):485-9 PMID: 4108619
  7. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses.
    J Virol. 1971 Nov;8(5):778-85 PMID: 4332144
  8. Characterization of the low-molecular-weight RNAs associated with the 70S RNA of Rous sarcoma virus.
    J Virol. 1973 Aug;12(2):334-42 PMID: 4355935
  9. Small RNAs of Rous sarcoma virus: characterization by two-dimensional polyacrylamide gel electrophoresis and fingerprint analysis.
    J Virol. 1973 Dec;12(6):1226-37 PMID: 4357505
  10. Mass and molecular composition of vesicular stomatitis virus: a scanning transmission electron microscopy analysis.
    J Virol. 1985 May;54(2):598-607 PMID: 2985822
  11. Expression of the Rous sarcoma virus pol gene by ribosomal frameshifting.
    Science. 1985 Dec 13;230(4731):1237-42 PMID: 2416054
  12. Structure and processing of the p2 region of avian sarcoma and leukemia virus gag precursor polyproteins.
    J Virol. 1986 Apr;58(1):50-8 PMID: 3005658
  13. Ubiquitin in avian leukosis virus particles.
    Virology. 1990 Jun;176(2):633-7 PMID: 2161153
  14. Properties of avian retrovirus particles defective in viral protease.
    J Virol. 1990 Oct;64(10):5076-92 PMID: 1697912
  15. Replication-competent retrovirus vectors for the transfer and expression of gene cassettes in avian cells.
    J Virol. 1991 Jul;65(7):3728-37 PMID: 2041092
  16. Appropriate in vivo expression of a muscle-specific promoter by using avian retroviral vectors for gene transfer [corrected].
    J Virol. 1992 Jun;66(6):3391-7 PMID: 1637416
  17. Cellular proteins bound to immunodeficiency viruses: implications for pathogenesis and vaccines.
    Science. 1992 Dec 18;258(5090):1935-8 PMID: 1470916
  18. Replication-competent retroviral vectors encoding alkaline phosphatase reveal spatial restriction of viral gene expression/transduction in the chick embryo.
    Mol Cell Biol. 1993 Apr;13(4):2604-13 PMID: 8455633
  19. Lipid composition and fluidity of the human immunodeficiency virus envelope and host cell plasma membranes.
    Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5181-5 PMID: 8389472
  20. Specific incorporation of cyclophilin A into HIV-1 virions.
    Nature. 1994 Nov 24;372(6504):359-62 PMID: 7969494
  21. Functional association of cyclophilin A with HIV-1 virions.
    Nature. 1994 Nov 24;372(6504):363-5 PMID: 7969495
  22. Differential proteolytic processing leads to multiple forms of the CA protein in avian sarcoma and leukemia viruses.
    J Virol. 1995 Oct;69(10):6430-8 PMID: 7666544
  23. Self-assembly in vitro of purified CA-NC proteins from Rous sarcoma virus and human immunodeficiency virus type 1.
    J Virol. 1995 Oct;69(10):6487-97 PMID: 7666550
  24. Analysis and localization of cyclophilin A found in the virions of human immunodeficiency virus type 1 MN strain.
    AIDS Res Hum Retroviruses. 1995 Sep;11(9):1003-6 PMID: 8554896
  25. Analysis of Rous sarcoma virus Gag protein by mass spectrometry indicates trimming by host exopeptidase.
    J Virol. 1996 May;70(5):3313-8 PMID: 8627817
  26. HIV-1 Gag protein associates with F-actin present in microfilaments.
    Virology. 1996 Jun 15;220(2):530-4 PMID: 8661406
  27. Cytoskeletal proteins inside human immunodeficiency virus type 1 virions.
    J Virol. 1996 Nov;70(11):7734-43 PMID: 8892894
  28. In vitro assembly of virus-like particles with Rous sarcoma virus Gag deletion mutants: identification of the p10 domain as a morphological determinant in the formation of spherical particles.
    J Virol. 1997 Jun;71(6):4425-35 PMID: 9151833
  29. Scanning transmission electron microscopy of nuclear structures.
    Methods Cell Biol. 1998;53:139-64 PMID: 9348508
  30. Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle.
    Curr Biol. 1997 Oct 1;7(10):729-38 PMID: 9368755
  31. Genetic determinants of Rous sarcoma virus particle size.
    J Virol. 1998 Jan;72(1):564-77 PMID: 9420260
  32. Particle size determinants in the human immunodeficiency virus type 1 Gag protein.
    J Virol. 1998 Jun;72(6):4667-77 PMID: 9573230
  33. Supramolecular organization of immature and mature murine leukemia virus revealed by electron cryo-microscopy: implications for retroviral assembly mechanisms.
    Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7299-304 PMID: 9636143
  34. Identification of retroviral late domains as determinants of particle size.
    J Virol. 1999 Mar;73(3):2309-20 PMID: 9971814
  35. Virus of avian myeloblastosis. XII. Chemical constitution.
    J Natl Cancer Inst. 1959 Jul;23(1):183-97 PMID: 13673277
  36. AVIAN VIRUS GROWTHS AND THEIR ETIOLOGIC AGENTS.
    Adv Cancer Res. 1963;7:1-127 PMID: 14153766
  37. Structural studies of avian myeloblastosis virus: comparison of polypeptides in virion and core component by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Virol. 1974 Feb;13(2):513-28 PMID: 4129794
  38. Generation of avian myeloblastosis virus structural proteins by proteolytic cleavage of a precursor polypeptide.
    J Mol Biol. 1975 Aug 15;96(3):471-93 PMID: 170408
  39. Novel p19-related protein in Rous-associated virus type 61: implications for avian gag gene order.
    J Virol. 1980 May;34(2):431-7 PMID: 6246274
  40. Proteins of Rous sarcoma virus.
    Virology. 1968 Sep;36(1):73-86 PMID: 4299391
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.8.7050-7055.1999
Email
[email protected]
Published
1999-08-00
Pages
7050-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC112795
Subset
IM
Grants
NCRR NIH HHS · P41 RR001777 · United States
NCI NIH HHS · R01 CA020081 · United States
NCI NIH HHS · CA20081 · United States
NCRR NIH HHS · P41-RR01777 · United States
External Links
PubMed source DOI: 10.1128/JVI.73.8.7050-7055.1999 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2