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PMID: 1637416 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Appropriate in vivo expression of a muscle-specific promoter by using avian retroviral vectors for gene transfer [corrected].

Journal of virology ·Vol. 66 ·No. 6 ·1992-06-00 ·Pages 3391-7

Petropoulos CJ, Payne W, Salter DW, Hughes SH

Abstract

The promoter regions of the chicken skeletal muscle alpha-actin (alpha sk-actin) and the cytoplasmic beta-actin genes were linked to the bacterial chloramphenicol acetyltransferase (CAT) gene. Replication-competent retroviral vectors were used to introduce these two actin/CAT cassettes into the chicken genome. Chickens infected with retroviruses containing the alpha sk-actin promoter expressed high levels of CAT activity in striated muscle (skeletal muscle and heart); much lower levels of CAT activity were produced in the other nonmuscle tissues. In contrast, chickens infected with retroviruses containing the beta-actin promoter linked to the CAT gene expressed low levels of CAT activity in many different tissue types and with no discernible tissue specificity. Data are presented to demonstrate that the high levels of CAT activity that were detected in the skeletal muscle of chickens infected with the retrovirus containing the alpha sk-actin promoter/CAT cassette were not due to preferential infectivity, integration, or replication of the retrovirus vector in the striated muscles of these animals.

MeSH Terms
Actins/genetics Animals Birds/microbiology Cells, Cultured Chick Embryo Chloramphenicol O-Acetyltransferase/genetics DNA, Viral/analysis Fibroblasts Genetic Vectors/genetics Organ Specificity Promoter Regions, Genetic/genetics Proviruses/chemistry Recombinant Fusion Proteins/genetics Retroviridae/genetics,growth & development Transfection/genetics Virus Replication
Chemicals
Actins DNA, Viral Recombinant Fusion Proteins Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Petropoulos C J
ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201.
Payne W
Salter D W
Hughes S H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.6.3391-3397.1992
Published
1992-06-00
Pages
3391-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241119
Subset
IM
Grants
NCI NIH HHS · N01-CO-74101 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1128/JVI.66.6.3391-3397.1992 PMC Full Text View journal details
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