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PMID: 10468601 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An enhancer element located downstream of the major glutamate dehydrogenase gene of Bacillus subtilis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 18 ·1999-08-31 ·Pages 10290-5

Belitsky BR, Sonenshein AL

Abstract

The rocG gene of Bacillus subtilis, encoding a catabolic glutamate dehydrogenase, is transcribed by SigL (sigma(54))-containing RNA polymerase and requires for its expression RocR, a member of the NtrC/NifA family of proteins that bind to enhancer-like elements, called upstream activating sequences (UAS). Unlike the case for other sigma(54)-dependent genes, rocG has no UAS; instead, its expression depends on a sequence located 1.5 kilobases downstream of the rocG promoter, beyond the end of the rocG coding region. The same sequence also serves as the UAS for the downstream rocABC operon and can activate rocG if moved upstream of its promoter. Furthermore, the activating sequence can be moved as far as 15 kilobases downstream of the rocG promoter and still retain partial activity.

MeSH Terms
Arginine/pharmacology Bacillus subtilis/enzymology,genetics Bacterial Proteins/metabolism Base Sequence DNA-Binding Proteins DNA-Directed RNA Polymerases/metabolism Enhancer Elements, Genetic Enzyme Induction/drug effects Enzyme Repression/drug effects Genes, Bacterial Glucose/pharmacology Glutamate Dehydrogenase/biosynthesis,genetics,metabolism Molecular Sequence Data Mutagenesis, Insertional Ornithine/pharmacology RNA Polymerase Sigma 54 Recombinant Fusion Proteins/metabolism Sequence Deletion Sigma Factor/metabolism Transcription, Genetic
Chemicals
Bacterial Proteins DNA-Binding Proteins Recombinant Fusion Proteins Sigma Factor Arginine Ornithine Glutamate Dehydrogenase DNA-Directed RNA Polymerases RNA Polymerase Sigma 54 Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Belitsky B R
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
Sonenshein A L
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.18.10290
Published
1999-08-31
Pages
10290-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC17881
Subset
IM
Grants
NIGMS NIH HHS · R01 GM036718 · United States
NIGMS NIH HHS · GM36718 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.18.10290 PMC Full Text View journal details
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