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PMID: 7596833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Analysis of the proteins and cis-acting elements regulating the stress-induced phage shock protein operon.

Nucleic acids research ·Vol. 23 ·No. 11 ·1995-06-11 ·Pages 2030-6

Weiner L, Brissette JL, Ramani N, Model P

Abstract

The phage shock protein operon (pspABCE) of Escherichia coli is strongly induced by adverse environmental conditions. Expression is controlled principally at the transcriptional level, and transcription is directed by the sigma factor sigma 54. PspB and PspC are required for high-level psp expression during osmotic shock, ethanol treatment and f1 infection, but heat-induced expression is independent of these proteins. We report here that the promoter region contains an upstream activation sequence (UAS) that is required for psp induction and has the enhancer-like ability to activate at a distance. A DNA-binding activity is detected in crude protein extracts that is dependent on the UAS and induced by heat shock. We further show that integration host factor (IHF) binds in vitro to a site between the UAS and sigma 54 recognition sequence. In bacteria lacking IHF, psp expression is substantially reduced in response to high temperature and ethanol. During osmotic shock in contrast, psp expression is only weakly stimulated by IHF, and IHF mutants can strongly induce the operon. The dependence of psp expression on IHF varies with the inducing condition, but does not correlate with dependence on PspB and PspC, indicating distinct, agent-specific activation mechanisms.

MeSH Terms
Bacterial Proteins/genetics,metabolism Base Sequence Escherichia coli/genetics,metabolism Gene Expression Regulation, Bacterial Heat-Shock Proteins/genetics,metabolism Molecular Sequence Data Operon/genetics
Chemicals
Bacterial Proteins Heat-Shock Proteins phage shock protein, Bacteria
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Weiner L
Laboratory of Genetics, Rockefeller University, New York, NY 10021, USA.
Brissette J L
Ramani N
Model P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.11.2030
Published
1995-06-11
Pages
2030-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306980
Subset
IM
Grants
NIAID NIH HHS · AI07233 · United States
NIGMS NIH HHS · GM 17152 · United States
Databases
GENBANK
X57560
External Links
PubMed source DOI: 10.1093/nar/23.11.2030 PMC Full Text View journal details
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