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PMID: 3045814 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Integration host factor interacts with the DNA replication enhancer of filamentous phage f1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 17 ·1988-09-00 ·Pages 6262-6

Greenstein D, Zinder ND, Horiuchi K

Abstract

We present data which show that the Escherichia coli integration host factor (IHF) is an activator of phage f1 DNA replication. Phage f1 poorly infects bacterial strains lacking IHF because IHF is required for efficient expression of F-pili, the receptor for f1 phage. However, when F- strains are transfected with f1 DNA the phage replicates in IHF mutants (himA, himD, or himA himD) at a rate of only 3% of that in wild-type bacteria. A plasmid dependent on the f1 replicon fails to transform IHF mutants. By gel retardation analysis, we show that IHF specifically binds to the origin of replication. DNase I "footprinting" experiments demonstrate that IHF binds to multiple sites within the replication enhancer sequence, a cis-acting, A + T-rich sequence that potentiates f1 DNA replication. Moreover, the effect of IHF mutation on f1 growth is suppressed by initiator protein (f1 gene II) mutations that restore efficient replication from origins that lack a functional replication enhancer sequence. This genetic evidence supports the conclusion that the replication enhancer sequence is the site of action of IHF.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence Coliphages/genetics,growth & development DNA Replication DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Escherichia coli/genetics Integration Host Factors Kinetics Mutation Replicon Transfection
Chemicals
Bacterial Proteins DNA-Binding Proteins Integration Host Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Greenstein D
Rockefeller University, New York, NY 10021.
Zinder N D
Horiuchi K
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.17.6262
Published
1988-09-00
Pages
6262-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281949
Subset
IM
Grants
NIAID NIH HHS · AI 07233 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.17.6262 PMC Full Text View journal details
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