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PMID: 10545444 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Activation of the Saccharomyces cerevisiae filamentation/invasion pathway by osmotic stress in high-osmolarity glycogen pathway mutants.

Genetics ·Vol. 153 ·No. 3 ·1999-11-00 ·Pages 1091-103

Davenport KD, Williams KE, Ullmann BD, Gustin MC

Abstract

Mitogen-activated protein kinase (MAPK) cascades are frequently used signal transduction mechanisms in eukaryotes. Of the five MAPK cascades in Saccharomyces cerevisiae, the high-osmolarity glycerol response (HOG) pathway functions to sense and respond to hypertonic stress. We utilized a partial loss-of-function mutant in the HOG pathway, pbs2-3, in a high-copy suppressor screen to identify proteins that modulate growth on high-osmolarity media. Three high-copy suppressors of pbs2-3 osmosensitivity were identified: MSG5, CAK1, and TRX1. Msg5p is a dual-specificity phosphatase that was previously demonstrated to dephosphorylate MAPKs in yeast. Deletions of the putative MAPK targets of Msg5p revealed that kss1delta could suppress the osmosensitivity of pbs2-3. Kss1p is phosphorylated in response to hyperosmotic shock in a pbs2-3 strain, but not in a wild-type strain nor in a pbs2-3 strain overexpressing MSG5. Both TEC1 and FRE::lacZ expressions are activated in strains lacking a functional HOG pathway during osmotic stress in a filamentation/invasion-pathway-dependent manner. Additionally, the cellular projections formed by a pbs2-3 mutant on high osmolarity are absent in strains lacking KSS1 or STE7. These data suggest that the loss of filamentation/invasion pathway repression contributes to the HOG mutant phenotype.

Keywords
NASA Discipline Cell Biology Non-NASA Center
MeSH Terms
Cloning, Molecular Genotype Glycerol/metabolism Glycogen/genetics Mitogen-Activated Protein Kinase Kinases/genetics,metabolism Mitogen-Activated Protein Kinases/metabolism Osmolar Concentration Saccharomyces cerevisiae/genetics,growth & development,physiology Saccharomyces cerevisiae Proteins Signal Transduction Sorbitol/pharmacology Suppression, Genetic/drug effects Transcription, Genetic
Chemicals
Saccharomyces cerevisiae Proteins Sorbitol Glycogen Mitogen-Activated Protein Kinases Mitogen-Activated Protein Kinase Kinases PBS2 protein, S cerevisiae Glycerol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Davenport K D
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005-1892, USA.
Williams K E
Ullmann B D
Gustin M C
Investigators
1 investigators, click to expand
McIntire L V
Rice U, Houston, TX
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44 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/153.3.1091
Published
1999-11-00
Pages
1091-103
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460814
Subset
IM
Grants
NIGMS NIH HHS · GMO-83262-7 · United States
External Links
PubMed source DOI: 10.1093/genetics/153.3.1091 PMC Full Text View journal details
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