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PMID: 7565726 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast RLM1 encodes a serum response factor-like protein that may function downstream of the Mpk1 (Slt2) mitogen-activated protein kinase pathway.

Molecular and cellular biology ·Vol. 15 ·No. 10 ·1995-10-00 ·Pages 5740-9

Watanabe Y, Irie K, Matsumoto K

Abstract

The MPK1 (SLT2) gene of Saccharomyces cerevisiae encodes a mitogen-activated protein kinase that is regulated by a kinase cascade whose known elements are Pkc1 (a homolog of protein kinase C), Bck1 (Slk1) (a homolog of MEK kinase), and the functionally redundant Mpk1 activators Mkk1 and Mkk2 (homologs of MEK). An activated mutation of MKK1, MKK1P386, inhibits growth when overexpressed. This growth-inhibitory effect was suppressed by the mpk1 delta mutation, suggesting that hyperactivation of the Mpk1 pathway is toxic to cells. To search for genes that interact with the Mpk1 pathway, we isolated both chromosomal mutations and dosage suppressor genes that ameliorate the growth-inhibitory effect of overexpressed Mkk1P386. One of the genes identified by the analysis of chromosomal mutations is RLM1 (resistance to lethality of MKK1P386 overexpression), which encodes a protein homologous to a conserved domain of the MADS (Mcm1, Agamous, Deficiens, and serum response factor) box family of transcription factors. Although rlm1 delta cells grow normally at any temperature, they display a caffeine-sensitive phenotype similar to that observed in mutants defective in BCK1, MKK1/MKK2, or MPK1. A gene fusion that provides Rlm1 with a transcriptional activation domain of Gal4 suppresses bck1 delta and mpk1 delta. A screening for dosage suppressors yielded the MSG5 genes, which encode a dual-specificity protein phosphatase. Our results suggest that Rlm1 functions as a transcription factor downstream of Mpk1 that is subject to activation by the Mpk1 mitogen-activated protein kinase pathway.

Related Genes
MKK1 RLM1 SLT2
MeSH Terms
Amino Acid Sequence Base Sequence Caffeine/pharmacology Calcium-Calmodulin-Dependent Protein Kinases/physiology Cloning, Molecular Fungal Proteins/biosynthesis,physiology Genes, Fungal/genetics Genetic Complementation Test MADS Domain Proteins MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Molecular Sequence Data Mutation Protein Serine-Threonine Kinases/genetics,physiology Protein Tyrosine Phosphatases/biosynthesis Protein-Tyrosine Kinases/genetics,physiology Recombinant Fusion Proteins/biosynthesis Restriction Mapping Saccharomyces cerevisiae/drug effects,enzymology,genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Deletion Sequence Homology, Amino Acid Signal Transduction/physiology Suppression, Genetic Transcription Factors/chemistry,genetics,physiology
Chemicals
Fungal Proteins MADS Domain Proteins RLM1 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors Caffeine Protein-Tyrosine Kinases Protein Serine-Threonine Kinases Calcium-Calmodulin-Dependent Protein Kinases Mitogen-Activated Protein Kinases SLT2 protein, S cerevisiae MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases MSG5 protein, S cerevisiae Protein Tyrosine Phosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Watanabe Y
Department of Molecular Biology, Faculty of Science, Nagoya University, Japan.
Irie K
Matsumoto K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.10.5740
Published
1995-10-00
Pages
5740-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230825
Subset
IM
Databases
GENBANK
D63340, U43281
External Links
PubMed source DOI: 10.1128/MCB.15.10.5740 PMC Full Text View journal details
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