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PMID: 1717994 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 20 ·1991-10-15 ·Pages 9006-10

Ninomiya-Tsuji J, Nomoto S, Yasuda H, Reed SI, Matsumoto K

Abstract

We have cloned two different human cDNAs that can complement cdc28 mutations of budding yeast Saccharomyces cerevisiae. One corresponds to a gene encoding human p34CDC2 kinase, and the other to a gene (CDK2; cell division kinase) that has not been characterized previously. The CDK2 protein is highly homologous to p34CDC2 kinase (65% identical) and more significantly is homologous to Xenopus Eg1 kinase (89% identical), suggesting that CDK2 is the human homolog of Eg1. The human CDC2 and CDK2 genes were both able to complement the inviability of a null allele of S. cerevisiae CDC28. This result indicates that the CDK2 protein has a biological activity closely related to the CDC28 and p34CDC2 kinases. However, CDK2 was unable to complement cdc2 mutants in fission yeast Schizosaccharomyces pombe under the condition where the human CDC2 gene could complement them. CDK2 mRNA appeared late in G1 or in early S phase, slightly before CDC2 mRNA, after growth stimulation in normal human fibroblast cells. These results suggest that in human cells, two different CDC2-like kinases may regulate the cell cycle at distinct stages.

Related Genes
CDC2 cdc28
MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence Blotting, Northern CDC2 Protein Kinase/genetics Cell Line Cloning, Molecular/methods DNA/genetics Genetic Complementation Test Humans Kinetics Molecular Sequence Data Mutation Plasmids Poly A/genetics RNA/genetics RNA, Messenger Restriction Mapping Saccharomyces cerevisiae/genetics Schizosaccharomyces/genetics Sequence Homology, Nucleic Acid Xenopus
Chemicals
RNA, Messenger Poly A RNA DNA CDC2 Protein Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ninomiya-Tsuji J
Department of Molecular Biology, DNAX Research Institute, Palo Alto, CA 94304.
Nomoto S
Yasuda H
Reed S I
Matsumoto K
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.20.9006
Published
1991-10-15
Pages
9006-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52640
Subset
IM
Grants
NIGMS NIH HHS · R01GM38328 · United States
Databases
GENBANK
M68520, S60062, S60067, S60069, S60071, S60073, S60076, S60079, S60081, S60380
External Links
PubMed source DOI: 10.1073/pnas.88.20.9006 PMC Full Text View journal details
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