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PMID: 2674687 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conservation of function and regulation within the Cdc28/cdc2 protein kinase family: characterization of the human Cdc2Hs protein kinase in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 9 ·1989-09-00 ·Pages 4064-8

Wittenberg C, Reed SI

Abstract

Whereas the Cdc28 protein kinase of the budding yeast Saccharomyces cerevisiae plays an essential role in cell cycle progression during the G1 interval, a function in the progression from the G2 interval into M phase has been inferred for its homologs, including the Cdc2Hs protein kinase of humans. To better understand these apparently disparate roles, we constructed a yeast strain in which the resident CDC28 gene was replaced by its human homolog, CDC2Hs. This transgenic yeast strain was able to perform the G1 functions attributed to the Cdc28 protein kinase, including the ability to grow and divide normally, to respond to environmental signals that induce G1 arrest, and to regulate the Cdc2Hs protein kinase appropriately in response to these signals.

MeSH Terms
Cell Cycle Chimera Humans Protein Kinases/genetics,physiology Saccharomyces cerevisiae/cytology,enzymology,genetics Transformation, Genetic
Chemicals
Protein Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wittenberg C
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Reed S I
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.9.4064-4068.1989
Published
1989-09-00
Pages
4064-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362473
Subset
IM
Grants
NIGMS NIH HHS · GM38328 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.9.4064-4068.1989 PMC Full Text View journal details
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