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PMID: 10579999 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2.

Genes & development ·Vol. 13 ·No. 22 ·1999-11-15 ·Pages 2928-33

Kamura T, Conrad MN, Yan Q, Conaway RC, Conaway JW

Abstract

The RING-H2 finger protein Rbx1 is a subunit of the related SCF (Skp1-Cdc53/Cul1-F-box protein) and von Hippel-Lindau (VHL) tumor suppressor (elongin BC-Cul2-VHL) E3 ubiquitin ligase complexes, where it functions as a component of Cdc53/Rbx1 and Cul2/Rbx1 modules that activate ubiquitination of target proteins by the E2 ubiquitin-conjugating enzymes Cdc34 and Ubc5. Here we demonstrate that the Cdc53/Rbx1 and Cul2/Rbx1 modules also activate conjugation of the ubiquitin-like protein Rub1 to Cdc53 and Cul2 by the dedicated E2 Rub1 conjugating enzyme Ubc12. Our findings identify Rbx1 as a common component of enzyme systems responsible for ubiquitin and Rub1 modification of target proteins.

MeSH Terms
Amino Acid Sequence Carrier Proteins/metabolism,physiology Cell Cycle Proteins/metabolism Cullin Proteins Fungal Proteins/metabolism,physiology Ligases Macromolecular Substances Molecular Sequence Data Peptide Synthases/physiology Protein Processing, Post-Translational Proteins/physiology Recombinant Fusion Proteins/metabolism SKP Cullin F-Box Protein Ligases Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid Tumor Suppressor Proteins Ubiquitin-Protein Ligases Ubiquitins Von Hippel-Lindau Tumor Suppressor Protein Zinc/metabolism
Chemicals
CUL2 protein, human Carrier Proteins Cdc53 protein, S cerevisiae Cell Cycle Proteins Cullin Proteins Fungal Proteins Macromolecular Substances Proteins RUB1 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Tumor Suppressor Proteins Ubiquitins SKP Cullin F-Box Protein Ligases Ubiquitin-Protein Ligases Von Hippel-Lindau Tumor Suppressor Protein Ligases Peptide Synthases Zinc
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kamura T
Howard Hughes Medical Institute (HHMI), Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA.
Conrad M N
Yan Q
Conaway R C
Conaway J W
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.22.2928
Published
1999-11-15
Pages
2928-33
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317157
Subset
IM
Grants
NIGMS NIH HHS · R01 GM041628 · United States
NIGMS NIH HHS · 2 ROI GM41628 · United States
External Links
PubMed source DOI: 10.1101/gad.13.22.2928 PMC Full Text View journal details
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