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PMID: 10618445 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 1 ·2000-01-04 ·Pages 489-94

Chisholm ST, Mahajan SK, Whitham SA, Yamamoto ML, Carrington JC

Abstract

The locus RTM1 is necessary for restriction of long-distance movement of tobacco etch virus in Arabidopsis thaliana without causing a hypersensitive response or inducing systemic acquired resistance. The RTM1 gene was isolated by map-based cloning. The deduced gene product is similar to the alpha-chain of the Artocarpus integrifolia lectin, jacalin, and to several proteins that contain multiple repeats of a jacalin-like sequence. These proteins comprise a family with members containing modular organizations of one or more jacalin repeat units and are implicated in defense against viruses, fungi, and insects.

MeSH Terms
Amino Acid Sequence Antiviral Agents/chemistry Arabidopsis/genetics Arabidopsis Proteins Chromosome Mapping Cloning, Molecular Genes, Plant Lectins/chemistry,genetics Molecular Sequence Data Plant Lectins Plant Proteins/chemistry,genetics Plant Viruses/pathogenicity Sequence Alignment
Chemicals
Antiviral Agents Arabidopsis Proteins Lectins Plant Lectins Plant Proteins RTM1 protein, Arabidopsis jacalin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chisholm S T
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
Mahajan S K
Whitham S A
Yamamoto M L
Carrington J C
References (35)
35 references, click to expand
  1. A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold.
    Nat Struct Biol. 1996 Jul;3(7):596-603 PMID: 8673603
  2. The myrosinase-binding protein from Brassica napus seeds possesses lectin activity and has a highly similar vegetatively expressed wound-inducible counterpart.
    Eur J Biochem. 1997 Dec 15;250(3):680-8 PMID: 9461290
  3. PLANT DISEASE RESISTANCE GENES.
    Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:575-607 PMID: 15012275
  4. Two jasmonate-inducible myrosinase-binding proteins from Brassica napus L. seedlings with homology to jacalin.
    Planta. 1998 Mar;204(3):295-304 PMID: 9530873
  5. The Rx gene from potato controls separate virus resistance and cell death responses.
    Plant Cell. 1999 May;11(5):781-92 PMID: 10330465
  6. Tagging of plant potyvirus replication and movement by insertion of beta-glucuronidase into the viral polyprotein.
    Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10208-12 PMID: 1438210
  7. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants.
    Transgenic Res. 1992 Nov;1(6):285-97 PMID: 1338696
  8. A similarity between viral defense and gene silencing in plants.
    Science. 1997 Jun 6;276(5318):1558-60 PMID: 18610513
  9. Distinct functions of capsid protein in assembly and movement of tobacco etch potyvirus in plants.
    EMBO J. 1994 Mar 15;13(6):1482-91 PMID: 7511101
  10. Long-distance movement factor: a transport function of the potyvirus helper component proteinase.
    Plant Cell. 1995 May;7(5):549-59 PMID: 7780307
  11. Suppression of long-distance movement of tobacco etch virus in a nonsusceptible host.
    J Virol. 1996 Apr;70(4):2556-61 PMID: 8642685
  12. Infectivity of turnip mosaic potyvirus cDNA clones and transcripts on the systemic host Arabidopsis thaliana and local lesion hosts.
    Virus Res. 1998 Jun;55(2):207-19 PMID: 9725673
  13. Jacalin: a jackfruit (Artocarpus heterophyllus) seed-derived lectin of versatile applications in immunobiological research.
    J Immunol Methods. 1998 Mar 15;212(2):193-211 PMID: 9672207
  14. Molecular genetics of plant disease resistance.
    Science. 1995 May 5;268(5211):661-7 PMID: 7732374
  15. Genome amplification and long-distance movement functions associated with the central domain of tobacco etch potyvirus helper component-proteinase.
    Virology. 1997 Feb 17;228(2):251-62 PMID: 9123832
  16. Selectable viruses and altered susceptibility mutants in Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):772-7 PMID: 9892709
  17. Characterization of cauliflower mosaic virus (CaMV) resistance in virus-resistant ecotypes of Arabidopsis.
    Mol Plant Microbe Interact. 1996 Dec;9(9):810-8 PMID: 8969529
  18. Capsid protein determinants involved in cell-to-cell and long distance movement of tobacco etch potyvirus.
    Virology. 1995 Feb 1;206(2):1007-16 PMID: 7856075
  19. Transcriptional and posttranscriptional plant gene silencing in response to a pathogen
    Science. 1998 Mar 27;279(5359):2113-5 PMID: 9516113
  20. Listening to the silent genes: transgene silencing, gene regulation and pathogen control.
    Trends Plant Sci. 1999 Sep;4(9):340-347 PMID: 10462766
  21. Identification and characterization of a locus (RTM1) that restricts long-distance movement of tobacco etch virus in Arabidopsis thaliana.
    Plant J. 1998 Apr;14(2):177-86 PMID: 9628015
  22. The amino acid sequences of jacalin and the Maclura pomifera agglutinin.
    FEBS Lett. 1991 May 6;282(2):382-4 PMID: 2037053
  23. The role of lectins in plant defence.
    Histochem J. 1995 Apr;27(4):253-71 PMID: 7635758
  24. In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration.
    Methods Mol Biol. 1998;82:259-66 PMID: 9664431
  25. Genetic evidence for an essential role for potyvirus CI protein in cell-to-cell movement.
    Plant J. 1998 May;14(4):393-400 PMID: 9670556
  26. 'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers.
    Nucleic Acids Res. 1988 Mar 11;16(5):1829-36 PMID: 2832831
  27. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  28. Nuclear transport of plant potyviral proteins.
    Plant Cell. 1990 Oct;2(10):987-98 PMID: 2136629
  29. VPg of tobacco etch potyvirus is a host genotype-specific determinant for long-distance movement.
    J Virol. 1997 Nov;71(11):8624-31 PMID: 9343220
  30. Variations in the VPg protein allow a potyvirus to overcome va gene resistance in tobacco.
    Virology. 1997 Oct 27;237(2):452-9 PMID: 9356356
  31. Myrosinase-binding proteins are derived from a large wound-inducible and repetitive transcript.
    Eur J Biochem. 1997 Feb 1;243(3):605-11 PMID: 9057822
  32. Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana.
    Plant Mol Biol. 1996 Nov;32(4):759-65 PMID: 8980528
  33. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
    Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PMID: 7984417
  34. Drosophila kelch is an oligomeric ring canal actin organizer.
    J Cell Biol. 1997 Aug 25;138(4):799-810 PMID: 9265647
  35. The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor.
    Cell. 1994 Sep 23;78(6):1101-15 PMID: 7923359
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.97.1.489
Published
2000-01-04
Pages
489-94
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC26690
Subset
IM
Grants
NIAID NIH HHS · R01 AI043288 · United States
NIAID NIH HHS · R21 AI043288 · United States
NIAID NIH HHS · R37 AI043288 · United States
NIAID NIH HHS · AI43288 · United States
Databases
GENBANK
AF191302
External Links
PubMed source DOI: 10.1073/pnas.97.1.489 PMC Full Text View journal details
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