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PMID: 10637312 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functions and functional domains of the GTPase Cdc42p.

Molecular biology of the cell ·Vol. 11 ·No. 1 ·2000-01-00 ·Pages 339-54

Kozminski KG, Chen AJ, Rodal AA, Drubin DG

Abstract

Cdc42p, a Rho family GTPase of the Ras superfamily, is a key regulator of cell polarity and morphogenesis in eukaryotes. Using 37 site-directed cdc42 mutants, we explored the functions and interactions of Cdc42p in the budding yeast Saccharomyces cerevisiae. Cytological and genetic analyses of these cdc42 mutants revealed novel and diverse phenotypes, showing that Cdc42p possesses at least two distinct essential functions and acts as a nodal point of cell polarity regulation in vivo. In addition, mapping the functional data for each cdc42 mutation onto a structural model of the protein revealed as functionally important a surface of Cdc42p that is distinct from the canonical protein-interacting domains (switch I, switch II, and the C terminus) identified previously in members of the Ras superfamily. This region overlaps with a region (alpha5-helix) recently predicted by structural models to be a specificity determinant for Cdc42p-protein interactions.

MeSH Terms
Amino Acid Sequence Binding Sites Humans Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Phenotype Protein Conformation cdc42 GTP-Binding Protein, Saccharomyces cerevisiae/chemistry,genetics,physiology
Chemicals
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kozminski K G
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202, USA.
Chen A J
Rodal A A
Drubin D G
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.1.339
Published
2000-01-00
Pages
339-54
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC14778
Subset
IM
Grants
NIGMS NIH HHS · R01 GM050399 · United States
NIGMS NIH HHS · GM50399 · United States
External Links
PubMed source DOI: 10.1091/mbc.11.1.339 PMC Full Text View journal details
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