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PMID: 10684246 Published · ppublish English Journal Article

Spatial separation of parental genomes in preimplantation mouse embryos.

The Journal of cell biology ·Vol. 148 ·No. 4 ·2000-02-21 ·Pages 629-34

Mayer W, Smith A, Fundele R, Haaf T

Abstract

We have used two different experimental approaches to demonstrate topological separation of parental genomes in preimplantation mouse embryos: mouse eggs fertilized with 5-bromodeoxyuridine (BrdU)-labeled sperm followed by detection of BrdU in early diploid embryos, and differential heterochromatin staining in mouse interspecific hybrid embryos. Separation of chromatin according to parental origin was preserved up to the four-cell embryo stage and then gradually disappeared. In F1 hybrid animals, genome separation was also observed in a proportion of somatic cells. Separate nuclear compartments during preimplantation development, when extreme chromatin remodelling occurs, and possibly in some differentiated cell types, may be associated with epigenetic reprogramming.

MeSH Terms
Animals Blastocyst/cytology,metabolism Bromodeoxyuridine/metabolism Cell Differentiation Cell Nucleus/genetics,metabolism Cells, Cultured Centromere/genetics,metabolism DNA/genetics,metabolism Diploidy Fathers Female Fibroblasts Genome Heterochromatin/genetics,metabolism Hybridization, Genetic In Situ Hybridization, Fluorescence Interphase Male Mice Mice, Inbred C3H Mice, Inbred C57BL Mothers Spermatozoa/cytology,metabolism Zygote/cytology,metabolism
Chemicals
Heterochromatin DNA Bromodeoxyuridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mayer W
Max-Planck-Institut für Molekulare Genetik, 14195 Berlin, Germany.
Smith A
Fundele R
Haaf T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.148.4.629
Published
2000-02-21
Pages
629-34
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2169371
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.148.4.629 PMC Full Text View journal details
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