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PMID: 2420175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Preferential somatic pairing between homologous heterochromatic regions of human chromosomes.

American journal of human genetics ·Vol. 38 ·No. 3 ·1986-03-00 ·Pages 319-29

Haaf T, Steinlein K, Schmid M

Abstract

The cytidine analog 5-azacytidine (5-azaC) induces an undercondensation of the heterochromatin in human chromosomes 1, 9, 15, 16, and Y when it is added in low concentrations to the late S-phase of growing lymphocyte cultures. In interphase nuclei, these heterochromatic regions are frequently somatically paired. The somatic pairing configurations are preserved up to metaphase stage in the 5-azaC-treated cultures and are thus susceptible to a direct microscopical examination. The statistical analysis of 1,000 somatic pairing configurations from 5-azaC-treated cells showed that the somatic pairing between the heterochromatic regions of homologous chromosomes is preferred over that between nonhomologous chromosomes.

MeSH Terms
Azacitidine/pharmacology Chromosomes, Human/drug effects,ultrastructure Chromosomes, Human, 1-3 Chromosomes, Human, 13-15 Chromosomes, Human, 16-18 Chromosomes, Human, 6-12 and X Heterochromatin/drug effects,ultrastructure Humans Karyotyping Lymphocytes/cytology,ultrastructure Male Mitosis Models, Genetic Y Chromosome
Chemicals
Heterochromatin Azacitidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Haaf T
Steinlein K
Schmid M
References (22)
22 references, click to expand
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1986-03-00
Pages
319-29
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1684778
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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