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PMID: 10708410 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Clink, a nanovirus-encoded protein, binds both pRB and SKP1.

Journal of virology ·Vol. 74 ·No. 7 ·2000-04-00 ·Pages 2967-72

Aronson MN, Meyer AD, Györgyey J, Katul L, Vetten HJ, Gronenborn B, Timchenko T

Abstract

Clink, a 20-kDa protein of faba bean necrotic yellows virus, a single-stranded DNA plant virus, interacts with pRB family members and a SKP1 homologue from Medicago sativa. An LxCxE motif and an F-box of Clink mediate the interactions with the respective proteins. The capacity of Clink to bind pRB correlates with its ability to stimulate viral replication. Interaction of a single protein with the cell cycle regulator pRB and SKP1, a constituent of the ubiquitin-protein turnover pathway, appears to be a novel feature. Hence, Clink may represent a new class of viral cell cycle modulators.

MeSH Terms
Amino Acid Motifs Amino Acid Sequence Base Sequence Binding Sites Carrier Proteins/chemistry,genetics,metabolism Cell Cycle Proteins/metabolism DNA Replication DNA, Viral/biosynthesis Molecular Sequence Data Mutagenesis, Site-Directed Protein Binding Retinoblastoma Protein/metabolism S-Phase Kinase-Associated Proteins Sequence Homology, Amino Acid Two-Hybrid System Techniques Viral Proteins/chemistry,genetics,metabolism
Chemicals
Carrier Proteins Cell Cycle Proteins Clink protein, faba bean necrotic yellows virus DNA, Viral Retinoblastoma Protein S-Phase Kinase-Associated Proteins Viral Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Aronson M N
Institut des Sciences Végétales, CNRS, 91198 Gif sur Yvette, France.
Meyer A D
Györgyey J
Katul L
Vetten H J
Gronenborn B
Timchenko T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.74.7.2967-2972.2000
Published
2000-04-00
Pages
2967-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC111794
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.74.7.2967-2972.2000 PMC Full Text View journal details
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