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PMID: 7647562 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells.

The Plant cell ·Vol. 7 ·No. 6 ·1995-06-00 ·Pages 705-19

Nagar S, Pedersen TJ, Carrick KM, Hanley-Bowdoin L, Robertson D

Abstract

Geminiviruses are plant DNA viruses that replicate through DNA intermediates in plant nuclei. The viral components required for replication are known, but no host factors have yet been identified. We used immunolocalization to show that the replication proteins of the geminivirus tomato golden mosaic virus (TGMV) are located in nuclei of terminally differentiated cells that have left the cell cycle. In addition, TGMV infection resulted in a significant accumulation of the host DNA synthesis protein proliferating cell nuclear antigen (PCNA). PCNA, an accessory factor for DNA polymerase delta, was not present at detectable levels in healthy differentiated cells. The TGMV replication protein AL1 was sufficient to induce accumulation of PCNA in terminally differentiated cells of transgenic plants. Analysis of the mechanism(s) whereby AL1 induces the accumulation of host replication machinery in quiescent plant cells will provide a unique opportunity to study plant DNA synthesis.

MeSH Terms
Base Sequence Cell Differentiation Geminiviridae/physiology Gene Expression Regulation, Viral Humans Molecular Sequence Data Oligodeoxyribonucleotides Plants, Genetically Modified Plants, Toxic Proliferating Cell Nuclear Antigen/genetics Tobacco/cytology,genetics,virology Virus Replication
Chemicals
Oligodeoxyribonucleotides Proliferating Cell Nuclear Antigen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nagar S
Department of Botany, North Carolina State University, Raleigh 27695-7612, USA.
Pedersen T J
Carrick K M
Hanley-Bowdoin L
Robertson D
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.7.6.705
Published
1995-06-00
Pages
705-19
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160820
Subset
IM
Grants
NIAID NIH HHS · AI08511 · United States
External Links
PubMed source DOI: 10.1105/tpc.7.6.705 PMC Full Text View journal details
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