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PMID: 10747096 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Fyn-binding protein (Fyb)/SLP-76-associated protein (SLAP), Ena/vasodilator-stimulated phosphoprotein (VASP) proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton.

The Journal of cell biology ·Vol. 149 ·No. 1 ·2000-04-03 ·Pages 181-94

Krause M, Sechi AS, Konradt M, Monner D, Gertler FB, Wehland J

Abstract

T cell receptor (TCR)-driven activation of helper T cells induces a rapid polarization of their cytoskeleton towards bound antigen presenting cells (APCs). We have identified the Fyn- and SLP-76-associated protein Fyb/SLAP as a new ligand for Ena/ vasodilator-stimulated phosphoprotein (VASP) homology 1 (EVH1) domains. Upon TCR engagement, Fyb/SLAP localizes at the interface between T cells and anti-CD3-coated beads, where Evl, a member of the Ena/VASP family, Wiskott-Aldrich syndrome protein (WASP) and the Arp2/3 complex are also found. In addition, Fyb/SLAP is restricted to lamellipodia of spreading platelets. In activated T cells, Fyb/SLAP associates with Ena/VASP family proteins and is present within biochemical complexes containing WASP, Nck, and SLP-76. Inhibition of binding between Fyb/SLAP and Ena/VASP proteins or WASP and the Arp2/3 complex impairs TCR-dependent actin rearrangement, suggesting that these interactions play a key role in linking T cell signaling to remodeling of the actin cytoskeleton.

MeSH Terms
Actin-Related Protein 2 Actin-Related Protein 3 Actins/antagonists & inhibitors,metabolism Adaptor Proteins, Signal Transducing Amino Acid Sequence Blood Platelets/cytology Carrier Proteins/antagonists & inhibitors,chemistry,metabolism Cell Adhesion Molecules/chemistry,metabolism Cloning, Molecular Cytoskeletal Proteins Cytoskeleton/metabolism Humans Lymphocyte Activation Microfilament Proteins Molecular Sequence Data Oncogene Proteins/metabolism Phosphoproteins/chemistry,metabolism Protein Binding Protein Isoforms/chemistry,metabolism Proteins/metabolism Pseudopodia/metabolism Receptor Aggregation Receptors, Antigen, T-Cell/metabolism Signal Transduction T-Lymphocytes/cytology,immunology,metabolism,ultrastructure Tumor Cells, Cultured Wiskott-Aldrich Syndrome Protein
Chemicals
ACTR2 protein, human ACTR3 protein, human Actin-Related Protein 2 Actin-Related Protein 3 Actins Adaptor Proteins, Signal Transducing Carrier Proteins Cell Adhesion Molecules Cytoskeletal Proteins FYB1 protein, human Microfilament Proteins Nck protein Oncogene Proteins Phosphoproteins Protein Isoforms Proteins Receptors, Antigen, T-Cell SLP-76 signal Transducing adaptor proteins WAS protein, human Wiskott-Aldrich Syndrome Protein vasodilator-stimulated phosphoprotein
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Krause M
Department of Cell Biology, Gesellschaft für Biotechnologische Forschung (GBF), D-38124 Braunschweig, Germany.
Sechi A S
Konradt M
Monner D
Gertler F B
Wehland J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.149.1.181
Published
2000-04-03
Pages
181-94
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2175102
Subset
IM
Grants
NIGMS NIH HHS · GM58801 · United States
Databases
GENBANK
AF198052
External Links
PubMed source DOI: 10.1083/jcb.149.1.181 PMC Full Text View journal details
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