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PMID: 10769038 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The glycosylphosphatidyl inositol-anchored adhesion molecule F3/contactin is required for surface transport of paranodin/contactin-associated protein (caspr).

The Journal of cell biology ·Vol. 149 ·No. 2 ·2000-04-17 ·Pages 491-502

Faivre-Sarrailh C, Gauthier F, Denisenko-Nehrbass N, Le Bivic A, Rougon G, Girault JA

Abstract

Paranodin/contactin-associated protein (caspr) is a transmembrane glycoprotein of the neurexin superfamily that is highly enriched in the paranodal regions of myelinated axons. We have investigated the role of its association with F3/contactin, a glycosylphosphatidyl inositol (GPI)-anchored neuronal adhesion molecule of the Ig superfamily. Paranodin was not expressed at the cell surface when transfected alone in CHO or neuroblastoma cells. Cotransfection with F3 resulted in plasma membrane delivery of paranodin, as analyzed by confocal microscopy and cell surface biotinylation. The region that mediates association with paranodin was mapped to the Ig domains of F3 by coimmunoprecipitation experiments. The association of paranodin with F3 allowed its recruitment to Triton X-100-insoluble microdomains. The GPI anchor of F3 was necessary, but not sufficient for surface expression of paranodin. F3-Ig, a form of F3 deleted of the fibronectin type III (FNIII) repeats, although GPI-linked and expressed at the cell surface, was not recovered in the microdomain fraction and was unable to promote cell surface targeting of paranodin. Thus, a cooperative effect between the GPI anchor, the FNIII repeats, and the Ig regions of F3 is required for recruitment of paranodin into lipid rafts and its sorting to the plasma membrane.

MeSH Terms
Animals CHO Cells COS Cells Cell Adhesion Molecules, Neuronal/chemistry,genetics,metabolism Cell Membrane/metabolism Contactins Cricetinae Fluorescent Antibody Technique, Indirect Glycosylphosphatidylinositols/metabolism Golgi Apparatus/metabolism,ultrastructure Humans Membrane Glycoproteins/chemistry,genetics,metabolism Models, Molecular Mutagenesis, Site-Directed Neuroblastoma Neuropeptides/chemistry,genetics,metabolism Protein Conformation Recombinant Fusion Proteins/chemistry,metabolism Repetitive Sequences, Amino Acid Sequence Deletion Transfection Tumor Cells, Cultured
Chemicals
Cell Adhesion Molecules, Neuronal Contactins Glycosylphosphatidylinositols Membrane Glycoproteins Neuropeptides Recombinant Fusion Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Faivre-Sarrailh C
Laboratoire de Génétique et Physiologie du Développement, UMR 6545 CNRS, IBDM, 13288 Marseille, France. [email protected]
Gauthier F
Denisenko-Nehrbass N
Le Bivic A
Rougon G
Girault J A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.149.2.491
Email
[email protected]
Published
2000-04-17
Pages
491-502
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2175151
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.149.2.491 PMC Full Text View journal details
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