DNA base compositions (GC content) of Saccharomyces species are reported and discussed. Several amendments of the four groups given by van der Walt are suggested, viz. the transfer of S. kluyveri to group 1, and of S. eupagycus, S. cidri, S. montanus, S. microellipsodes and S. florentinus to group 2. The synonomy of S. amurcae and S. cidri is suggested. The DNA base compositions revealed two possible pairs of sibling species: S. elegans and S. bailii, with a difference in GC content of 4.1%; S. dairensis and S. servazzii with a difference in GC content of ca. 3%. S. mrakii had a GC content of 47.3-48.5% the highest encountered in this genus and similar to that of Kluyveromyces thermotolerans.
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