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PMID: 10869088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A PhoP-regulated outer membrane protease of Salmonella enterica serovar typhimurium promotes resistance to alpha-helical antimicrobial peptides.

Journal of bacteriology ·Vol. 182 ·No. 14 ·2000-07-00 ·Pages 4077-86

Guina T, Yi EC, Wang H, Hackett M, Miller SI

Abstract

The outer membrane protein contents of Salmonella enterica serovar Typhimurium strains with PhoP/PhoQ regulon mutations were compared by two-dimensional gel electrophoresis. At least 26 species of outer membrane proteins (OMPs) were identified as being regulated by PhoP/PhoQ activation. One PhoP/PhoQ-activated OMP was identified by semiautomated tandem mass spectrometry coupled with electronic database searching as PgtE, a member of the Escherichia coli OmpT and Yersinia pestis Pla family of outer membrane proteases. Salmonella PgtE expression promoted resistance to alpha-helical cationic antimicrobial peptides (alpha-CAMPs). Strains expressing PgtE cleaved C18G, an 18-residue alpha-CAMP present in culture medium, indicating that protease activity is likely to be the mechanism of OmpT-mediated resistance to alpha-CAMPs. PhoP/PhoQ did not regulate the transcription or export of PgtE, indicating that another PhoP/PhoQ-dependent mechanism is required for PgtE outer membrane localization. PgtE is a posttranscriptionally regulated component of the PhoP/PhoQ regulon that contributes to Salmonella resistance to innate immunity.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/pharmacology Bacterial Proteins/genetics Drug Resistance, Microbial Endopeptidases/genetics Gene Expression Regulation, Bacterial Mass Spectrometry Microbial Sensitivity Tests Molecular Sequence Data Peptide Fragments/chemistry Peptides/pharmacology Protein Structure, Secondary Salmonella typhimurium/drug effects,enzymology,genetics Sequence Analysis, DNA Sequence Analysis, Protein
Chemicals
Anti-Bacterial Agents Bacterial Proteins Peptide Fragments Peptides PhoP protein, Bacteria Endopeptidases PgtE protein, Salmonella enterica
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Guina T
Departments of Microbiology, University of Washington, Seattle 98195, USA.
Yi E C
Wang H
Hackett M
Miller S I
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.14.4077-4086.2000
Published
2000-07-00
Pages
4077-86
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94595
Subset
IM
Grants
NIAID NIH HHS · R01 AI030479 · United States
NIAID NIH HHS · R01 AI30479 · United States
Databases
GENBANK
AF239770
External Links
PubMed source DOI: 10.1128/JB.182.14.4077-4086.2000 PMC Full Text View journal details
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