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PMID: 8892844 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcriptional regulation of Salmonella virulence: a PhoQ periplasmic domain mutation results in increased net phosphotransfer to PhoP.

Journal of bacteriology ·Vol. 178 ·No. 21 ·1996-11-00 ·Pages 6369-73

Gunn JS, Hohmann EL, Miller SI

Abstract

A mutation in the phoP/phoQ locus (pho-24) that results in unregulated expression of PhoP-activated genes (phenotype PhoP constitutive [PhoP(c)]) was mapped to phoQ. Change of a Thr to Ile at position 48 of PhoQ was responsible for the PhoP(c) phenotype (attenuation of mouse virulence, defects in epithelial cell invasion, and macrophage spacious phagosome formation). PhoP phosphorylation by membrane extracts required PhoQ, and PhoQ Ile-48-containing membranes demonstrated increased net phosphorylation of PhoP.

MeSH Terms
Amino Acid Sequence Animals Bacterial Proteins/genetics Gene Expression Regulation, Bacterial Mice Molecular Sequence Data Phosphorylation Salmonella/genetics,pathogenicity Transcription Factors/genetics Transcription, Genetic Virulence
Chemicals
Bacterial Proteins PhoQ protein, Bacteria Transcription Factors PhoP protein, Bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gunn J S
Department of Medicine, University of Washington, Seattle 98195, USA.
Hohmann E L
Miller S I
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.21.6369-6373.1996
Published
1996-11-00
Pages
6369-73
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178515
Subset
IM
Grants
NIAID NIH HHS · AI30479 · United States
External Links
PubMed source DOI: 10.1128/jb.178.21.6369-6373.1996 PMC Full Text View journal details
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