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PMID: 10872319 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli.

The EMBO journal ·Vol. 2 ·No. 8 ·1983-00-00 ·Pages 1271-4

von Wilcken-Bergmann B, Koenen M, Griesser HW, Müller-Hill B

Abstract

An active gene has been constructed which produces a chimera consisting of the N-terminal domain of the gal repressor and all but the first five residues of beta-galactosidase. Seventy two residues of gal repressor fused to beta-galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro.

MeSH Terms
Amino Acid Sequence Artificial Gene Fusion Base Sequence DNA, Bacterial Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Molecular Sequence Data Operator Regions, Genetic Operon Repressor Proteins/genetics beta-Galactosidase/genetics
Chemicals
DNA, Bacterial Escherichia coli Proteins Galactose repressor proteins Repressor Proteins beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
von Wilcken-Bergmann B
Institut für Genetik, Universität zu Köln, FRG.
Koenen M
Griesser H W
Müller-Hill B
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21 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1271-4
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555271
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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