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PMID: 1093184 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A ribonucleoprotein precursor of both the 30S and 50S ribosomal sunbunits of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 4 ·1975-04-00 ·Pages 1533-7

Duncan MJ, Gorini L

Abstract

A ribonucleoprotein particle (46S) has been isolated from [3H]uridine pulse-labeled cultures of E. Coli AB301/105. Evidence from pulse chase experiments and from protein analysis suggested that this particle may give rise to both the 30S and 50S ribosomal subunits. Direct deproteinization of the particle yielded 30S RNA, while deproteinization after treatment with a crude RNase III preparation yielded products similar to 23S and 16S RNA. This result is consistent with the idea that the 46S ribonucleoprotein is the in vivo counterpart of 30S RNA, which is the in vitro product obtained after phenol extraction.

MeSH Terms
Bacterial Proteins/analysis Cell Fractionation Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism Mutation Nucleoproteins/metabolism RNA, Bacterial/metabolism RNA, Ribosomal/metabolism Ribosomes/metabolism,ultrastructure Uridine/metabolism
Chemicals
Bacterial Proteins Nucleoproteins RNA, Bacterial RNA, Ribosomal Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Duncan M J
Gorini L
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.4.1533
Published
1975-04-00
Pages
1533-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432571
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.4.1533 PMC Full Text View journal details
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