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PMID: 10948264 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

PBF-2 is a novel single-stranded DNA binding factor implicated in PR-10a gene activation in potato.

The Plant cell ·Vol. 12 ·No. 8 ·2000-08-00 ·Pages 1477-89

Desveaux D, Després C, Joyeux A, Subramaniam R, Brisson N

Abstract

Elicitor-induced activation of the potato pathogenesis-related gene PR-10a requires a 30-bp promoter sequence termed the ERE (elicitor response element) that is bound by the nuclear factor PBF-2 (PR-10a binding factor 2). In this study, PBF-2 has been purified to near homogeneity from elicited tubers through a combination of anion-exchange and DNA affinity chromatography. Evidence demonstrates that inactive PBF-2 is stored in the nuclei of fresh tubers and becomes available for binding to the ERE upon elicitation. A protein with an apparent molecular mass of 24 kD (p24) is a DNA binding component of PBF-2. A cDNA encoding p24 has been cloned and encodes a novel protein with a potential transcriptional activation domain that could also act as a single-stranded DNA binding domain. Both PBF-2 and the cDNA-encoded protein bind with high affinity to the single-stranded form of the ERE in a sequence-specific manner. The inverted repeat sequence of the ERE, TGACAnnnnTGTCA, is critical for binding of this factor in vitro and for PR-10a expression in vivo, supporting the role of PBF-2 as a transcriptional regulator.

MeSH Terms
Amino Acid Sequence Antibodies/immunology,pharmacology Base Sequence Binding Sites Cell Nucleus/chemistry,drug effects,metabolism Chromatography, Affinity Cloning, Molecular DNA, Single-Stranded/genetics,metabolism DNA-Binding Proteins/chemistry,genetics,isolation & purification,metabolism Gene Expression Regulation, Plant/drug effects Genes, Plant/genetics Genes, Reporter/genetics Models, Genetic Molecular Sequence Data Molecular Weight Mutation Nuclear Proteins/chemistry,genetics,isolation & purification,metabolism Peptides/chemistry,genetics Plant Growth Regulators/pharmacology Plant Proteins Protein Binding/drug effects Recombinant Proteins/chemistry,genetics,isolation & purification,metabolism Response Elements/genetics Solanum tuberosum/cytology,drug effects,genetics Substrate Specificity Trans-Activators/chemistry,genetics,isolation & purification,metabolism Transcriptional Activation/drug effects
Chemicals
Antibodies DNA, Single-Stranded DNA-Binding Proteins Nuclear Proteins PBF-2 protein, Solanum tuberosum Peptides Plant Growth Regulators Plant Proteins Recombinant Proteins Trans-Activators polyglutamine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Desveaux D
Department of Biochemistry, Université de Montréal, Montréal, Québec, Canada H3C 3J7.
Després C
Joyeux A
Subramaniam R
Brisson N
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.12.8.1477
Published
2000-08-00
Pages
1477-89
Language
English
Region
England
NLM ID
9208688
PMCID
PMC149117
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.12.8.1477 PMC Full Text View journal details
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