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PMID: 8896461 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Activation of the CaMV as-1 cis-element by salicylic acid: differential DNA-binding of a factor related to TGA1a.

The EMBO journal ·Vol. 15 ·No. 20 ·1996-10-15 ·Pages 5679-89

Jupin I, Chua NH

Abstract

Transcription from the as-1 element of the cauliflower mosaic virus is induced by salicylic acid (SA), an endogenous signal involved in plant defence responses. Electrophoretic mobility shift assays demonstrated that the binding of a tobacco cellular factor, named SARP, correlates with the SA-induced activation of transcription. SARP was shown to contain proteins immunologically related to TGA1a, a transcription factor previously cloned for its ability to bind to the as-1 element. The molecular mass of SARP was estimated to be 40 kDa by South-Western experiments. Treatment of the extracts with dissociating agents led to an increase in the DNA binding activity, suggesting the presence of an inhibitor that sequesters SARP. The DNA binding activity appeared sensitive to phosphatase treatment, suggesting a role for phosphorylation in the SA-induced gene activation. These results represent an analysis of immediate early response to SA and potentially elucidate the events of the SA signal transduction pathway.

MeSH Terms
Basic-Leucine Zipper Transcription Factors Blotting, Southern Blotting, Western Cycloheximide/pharmacology DNA/metabolism DNA-Binding Proteins/chemistry,metabolism Molecular Weight Phosphoric Monoester Hydrolases/metabolism Plant Growth Regulators/pharmacology Plant Proteins/metabolism Plants, Toxic Salicylates/pharmacology Salicylic Acid Tobacco Transcription Factors/metabolism Viral Proteins/genetics
Chemicals
Basic-Leucine Zipper Transcription Factors DNA-Binding Proteins P1 protein, Cauliflower mosaic virus Plant Growth Regulators Plant Proteins Salicylates TGA1a protein, Nicotiana tabacum Transcription Factors Viral Proteins DNA Cycloheximide Phosphoric Monoester Hydrolases Salicylic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jupin I
Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10021, USA.
Chua N H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-10-15
Pages
5679-89
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452312
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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