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PMID: 7811965 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The ocs element in the soybean GH2/4 promoter is activated by both active and inactive auxin and salicylic acid analogues.

Plant molecular biology ·Vol. 26 ·No. 4 ·1994-11-00 ·Pages 1055-64

Ulmasov T, Hagen G, Guilfoyle T

Abstract

The octopine synthase (ocs or ocs-like) element has been previously reported to be responsive to the plant hormones, auxin, salicylic acid, and methyl jasmonate. Using transient assays with carrot protoplasts, we have demonstrated that an ocs element from the soybean auxin-inducible GH2/4 promoter is not only activated by strong auxins (i.e., 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, alpha-naphthalene acetic acid) and salicylic acid, but also by weak auxin analogues (beta-naphthalene acetic acid), inactive auxin analogs (i.e., 2,3-dichlorophenoxyacetic acid, 2,4,6-trichlorophenoxyacetic acid), and inactive salicylic acid analogs (3-hydroxybenzoic acid and 4-hydroxybenzoic acid). Our results indicate that the ocs element in the GH2/4 promoter is not selectively induced by plant hormones and might function similarly to tandem AP-1 sites in some animal glutathione S-transferase (GST) genes. The ocs element, like the AP-1 sites in animal GST promoters, may be induced not only by certain hormones but also by some non-hormonal stress-inducing or electrophilic agents.

MeSH Terms
Amino Acid Oxidoreductases/genetics Base Sequence Daucus carota/genetics Gene Expression Regulation, Plant Genes, Reporter Indoleacetic Acids/pharmacology Molecular Sequence Data Plant Growth Regulators/pharmacology Promoter Regions, Genetic/genetics Salicylates/pharmacology Soybeans/genetics
Chemicals
Indoleacetic Acids Plant Growth Regulators Salicylates Amino Acid Oxidoreductases D-octopine dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ulmasov T
Department of Biochemistry, University of Missouri, Columbia 65211.
Hagen G
Guilfoyle T
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47 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00040688
Published
1994-11-00
Pages
1055-64
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00040688 View journal details
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