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PMID: 8507830 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene STH-2 in potato.

Plant molecular biology ·Vol. 22 ·No. 2 ·1993-05-00 ·Pages 279-91

Matton DP, Prescott G, Bertrand C, Camirand A, Brisson N

Abstract

We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene consisting of 1015 bp of 5'-flanking sequence and part of the first exon of STH-2 fused to the bacterial beta-glucuronidase gene was highly-expressed in tubers of transgenic potato plants after wounding and elicitor treatments. The levels of activity observed in these transgenic plants parallel those observed for the accumulation of STH-2 mRNAs under similar conditions. This indicates that cis-acting elements necessary for the proper activation of the gene are present within 1 kb of 5'-flanking sequences. Functional analysis of 5' deletions of the STH-2/GUS constructs by transient expression in leaf protoplasts revealed the presence of an upstream regulatory sequence between -135 and -52 which contains a TGAC motif, and a possible negative regulatory region between -52 and -28. A factor present in nuclear extracts of wounded potato tubers was found to bind specifically to nucleotides located between -135 to -105, suggesting that this region contains important cis-regulatory elements.

Related Genes
STH-2 STH-21
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Mutational Analysis DNA-Binding Proteins/isolation & purification Gene Expression Regulation Genes, Plant/genetics Glucuronidase/biosynthesis Molecular Sequence Data Plant Proteins/biosynthesis,genetics Plants, Genetically Modified/genetics Recombinant Fusion Proteins/biosynthesis Regulatory Sequences, Nucleic Acid/genetics Restriction Mapping Sequence Deletion Sequence Homology, Nucleic Acid Solanum tuberosum/genetics Transformation, Genetic
Chemicals
DNA-Binding Proteins Plant Proteins Recombinant Fusion Proteins pathogenesis-related proteins, plant Glucuronidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Matton D P
Department of Biochemistry, Université de Montréal, Québec, Canada.
Prescott G
Bertrand C
Camirand A
Brisson N
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00014935
Published
1993-05-00
Pages
279-91
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
M29041, M29042
External Links
PubMed source DOI: 10.1007/BF00014935 View journal details
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