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PMID: 1095551 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of a nontransmissible antibiotic resistance plasmid by transductional shortening of R factor RP1.

Journal of bacteriology ·Vol. 123 ·No. 1 ·1975-07-00 ·Pages 20-7

Shipley PL, Olsen RH

Abstract

A plasmid segregant carrying tetracycline and carbenicillin resistance markers has been isolated from R factor RP1 by transductional shortening with phage P22. The new plasmid RP1-S2, which has a molecular weight of 23 times 10-6, has lost the transfer, phage sensitivity, and neomycin resistance functions of RP1. It combines readily with a W group plasmid, R388, to form a transmissible carbenicillin and trimethoprim resistance plasmid, RWP1.

MeSH Terms
Carbenicillin/pharmacology Coliphages/metabolism DNA, Bacterial/isolation & purification,metabolism Escherichia coli/drug effects,metabolism Extrachromosomal Inheritance Penicillin Resistance Plasmids R Factors Salmonella Phages/metabolism Salmonella typhimurium/drug effects,metabolism Tetracycline/pharmacology Transduction, Genetic
Chemicals
DNA, Bacterial Tetracycline Carbenicillin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shipley P L
Olsen R H
References (30)
30 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.123.1.20-27.1975
Published
1975-07-00
Pages
20-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235686
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.123.1.20-27.1975 PMC Full Text View journal details
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