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PMID: 10974003 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two cell adhesion molecules, nectin and cadherin, interact through their cytoplasmic domain-associated proteins.

The Journal of cell biology ·Vol. 150 ·No. 5 ·2000-09-04 ·Pages 1161-76

Tachibana K, Nakanishi H, Mandai K, Ozaki K, Ikeda W, Yamamoto Y, Nagafuchi A, Tsukita S, Takai Y

Abstract

We have found a new cell-cell adhesion system at cadherin-based cell-cell adherens junctions (AJs) consisting of at least nectin and l-afadin. Nectin is a Ca(2+)-independent homophilic immunoglobulin-like adhesion molecule, and l-afadin is an actin filament-binding protein that connects the cytoplasmic region of nectin to the actin cytoskeleton. Both the trans-interaction of nectin and the interaction of nectin with l-afadin are necessary for their colocalization with E-cadherin and catenins at AJs. Here, we examined the mechanism of interaction between these two cell-cell adhesion systems at AJs by the use of alpha-catenin-deficient F9 cell lines and cadherin-deficient L cell lines stably expressing their various components. We showed here that nectin and E-cadherin were colocalized through l-afadin and the COOH-terminal half of alpha-catenin at AJs. Nectin trans-interacted independently of E-cadherin, and the complex of E-cadherin and alpha- and beta-catenins was recruited to nectin-based cell-cell adhesion sites through l-afadin without the trans-interaction of E-cadherin. Our results indicate that nectin and cadherin interact through their cytoplasmic domain-associated proteins and suggest that these two cell-cell adhesion systems cooperatively organize cell-cell AJs.

MeSH Terms
Animals Base Sequence COS Cells Cadherins/chemistry,physiology Cell Adhesion Molecules/chemistry,genetics,physiology Cell Line Coculture Techniques Cytoplasm/physiology,ultrastructure Cytoskeletal Proteins/physiology DNA, Complementary Humans Intercellular Junctions/physiology,ultrastructure Kinesins L Cells Mice Microfilament Proteins/physiology Molecular Sequence Data Myosins Nectins Receptors, Virus/chemistry,genetics,physiology Recombinant Proteins/chemistry,metabolism Transfection alpha Catenin
Chemicals
AFDN protein, human Afdn protein, mouse CTNNA1 protein, human Cadherins Cell Adhesion Molecules Ctnna1 protein, mouse Cytoskeletal Proteins DNA, Complementary Microfilament Proteins Nectins Receptors, Virus Recombinant Proteins afadin alpha Catenin Myosins Kinesins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Tachibana K
Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Japan.
Nakanishi H
Mandai K
Ozaki K
Ikeda W
Yamamoto Y
Nagafuchi A
Tsukita S
Takai Y
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.150.5.1161
Published
2000-09-04
Pages
1161-76
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2175253
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.150.5.1161 PMC Full Text View journal details
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