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PMID: 11069885 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Genetic and biochemical characterization of dTOR, the Drosophila homolog of the target of rapamycin.

Genes & development ·Vol. 14 ·No. 21 ·2000-11-01 ·Pages 2689-94

Oldham S, Montagne J, Radimerski T, Thomas G, Hafen E

Abstract

The adaptation of growth in response to nutritional changes is essential for the proper development of all organisms. Here we describe the identification of the Drosophila homolog of the target of rapamycin (TOR), a candidate effector for nutritional sensing. Genetic and biochemical analyses indicate that dTOR impinges on the insulin signaling pathway by autonomously affecting growth through modulating the activity of dS6K. However, in contrast to other components in the insulin signaling pathway, partial loss of dTOR function preferentially reduces growth of the endoreplicating tissues. These results are consistent with dTOR residing on a parallel amino acid sensing pathway.

MeSH Terms
Amino Acids/metabolism Animals Carrier Proteins Cell Cycle Proteins Drosophila Proteins Drosophila melanogaster/enzymology,genetics,growth & development,physiology Energy Metabolism/drug effects,genetics Fungal Proteins/physiology Genes, Lethal Insect Proteins/analysis,genetics,physiology Insulin/physiology Intracellular Signaling Peptides and Proteins Larva Peptide Initiation Factors Phosphatidylinositol 3-Kinases/physiology Phosphoproteins/physiology Phosphotransferases (Alcohol Group Acceptor)/analysis,genetics,physiology Protein Kinases Recombinant Fusion Proteins/metabolism Ribosomal Protein S6 Kinases/physiology Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins Signal Transduction Sirolimus/pharmacology Species Specificity TOR Serine-Threonine Kinases
Chemicals
Amino Acids Carrier Proteins Cell Cycle Proteins Drosophila Proteins Fungal Proteins Insect Proteins Insulin Intracellular Signaling Peptides and Proteins Peptide Initiation Factors Phosphoproteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Thor protein, Drosophila Protein Kinases Phosphotransferases (Alcohol Group Acceptor) target of rapamycin protein, Drosophila TOR1 protein, S cerevisiae TOR2 protein, S cerevisiae Ribosomal Protein S6 Kinases TOR Serine-Threonine Kinases Sirolimus
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Oldham S
Zoologisches Institut, Universität Zürich, 8057 Zürich, Switzerland.
Montagne J
Radimerski T
Thomas G
Hafen E
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.845700
Published
2000-11-01
Pages
2689-94
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317036
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.845700 PMC Full Text View journal details
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