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PMID: 11095746 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

MHC I-dependent antigen presentation is inhibited by poliovirus protein 3A.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 25 ·2000-12-05 ·Pages 13790-5

Deitz SB, Dodd DA, Cooper S, Parham P, Kirkegaard K

Abstract

The effects of poliovirus 3A protein expression and poliovirus infection on the presentation of hepatitis C virus antigens in cultured chimpanzee cells were examined. Expression of poliovirus 3A protein inhibits protein secretion when expressed in isolation and was sufficient to protect chimpanzee cells from lysis by hepatitis C virus-specific cytotoxic T cells in standard (51)Cr-release assays. Poliovirus infection also inhibited antigen presentation, as determined by decreased cytotoxic T cell activation. A mutation in 3A that abrogates the inhibition of protein secretion also abolished the effects of poliovirus on antigen presentation. These results demonstrate that the inhibition of secretion observed in poliovirus-infected cells substantially reduces the presentation of new antigens on the cell surface. These observations may reflect a general mechanism by which nonenveloped viruses such as poliovirus and other viruses that do not require a functional protein secretory apparatus can evade detection by the cellular immune response.

MeSH Terms
Animals Cell Line Histocompatibility Antigens Class I/biosynthesis,immunology Pan troglodytes T-Lymphocytes, Cytotoxic/immunology Viral Core Proteins/physiology
Chemicals
3A protein, Poliovirus Histocompatibility Antigens Class I Viral Core Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Deitz S B
Departments of Microbiology and Immunology, and Structural Biology, Stanford University, School of Medicine, Stanford, CA 94305, USA.
Dodd D A
Cooper S
Parham P
Kirkegaard K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.250483097
Published
2000-12-05
Pages
13790-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC17654
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.250483097 PMC Full Text View journal details
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