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PMID: 7889939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inhibition of cellular protein secretion by poliovirus proteins 2B and 3A.

The EMBO journal ·Vol. 14 ·No. 5 ·1995-03-01 ·Pages 894-907

Doedens JR, Kirkegaard K

Abstract

Poliovirus RNA replication occurs on the surface of membranous vesicles that proliferate throughout the cytoplasm of the infected cell. Since at least some of these vesicles are thought to originate within the secretory pathway of the host cell, we examined the effect of poliovirus infection on protein transport through the secretory pathway. We found that transport of both plasma membrane and secretory proteins was inhibited by poliovirus infection early in the infectious cycle. Transport inhibition did not require viral RNA replication or the inhibition of host cell translation by poliovirus. The viral proteins 2B and 3A were each sufficient to inhibit transport in the absence of viral infection. The intracellular localization of a secreted protein in the presence of 3A with the endoplasmic reticulum suggested that 3A directly blocks transport from the endoplasmic reticulum to the Golgi apparatus.

MeSH Terms
Cell Line Cell Membrane Permeability Cytoplasm/chemistry Endocytosis Endoplasmic Reticulum/metabolism Glycosylation Golgi Apparatus/metabolism Humans Hygromycin B/metabolism,pharmacology Isomerases/analysis Membrane Glycoproteins Plasmids Poliovirus/metabolism Protein Biosynthesis/drug effects Protein Disulfide-Isomerases RNA, Viral/biosynthesis Recombinant Fusion Proteins/metabolism Transfection Viral Envelope Proteins/genetics,metabolism Viral Nonstructural Proteins/physiology alpha 1-Antitrypsin/genetics,metabolism
Chemicals
G protein, vesicular stomatitis virus Membrane Glycoproteins RNA, Viral Recombinant Fusion Proteins Viral Envelope Proteins Viral Nonstructural Proteins alpha 1-Antitrypsin Hygromycin B Isomerases Protein Disulfide-Isomerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Doedens J R
Department of Molecular, Cellular and Developmental Biology, Howard Hughes Medical Institute, University of Colorado, Boulder 80309-0347.
Kirkegaard K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-03-01
Pages
894-907
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398162
Subset
IM
Grants
NIAID NIH HHS · AI-25166 · United States
External Links
PubMed source PMC Full Text View journal details
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