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PMID: 2538648 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo.

Journal of virology ·Vol. 63 ·No. 4 ·1989-04-00 ·Pages 1651-60

Jang SK, Davies MV, Kaufman RJ, Wimmer E

Abstract

Expression vectors that yield mono-, di-, and tricistronic mRNAs upon transfection of COS-1 cells were used to assess the influence of the 5' nontranslated regions (5'NTRs) on translation of reporter genes. A segment of the 5'NTR of encephalomyocarditis virus (EMCV) allowed translation of an adjacent downstream reporter gene (CAT) regardless of its position in the mRNAs. A deletion in the EMCV 5'NTR abolishes this effect. Poliovirus infection completely inhibits translation of the first cistron of a dicistronic mRNA that is preceded by the capped globin 5'NTR, whereas the second cistron preceded by the EMCV 5'NTR is still translated. We conclude that the EMCV 5'NTR contains an internal ribosomal entry site that allows cap-independent initiation of translation. mRNA containing the adenovirus tripartite leader is also resistant to inhibition of translation by poliovirus.

MeSH Terms
Animals Cell Line Chloramphenicol O-Acetyltransferase/genetics Cloning, Molecular Encephalomyocarditis virus/genetics Genes Peptide Chain Initiation, Translational Poliovirus/genetics RNA, Messenger/genetics RNA, Viral/genetics Regulatory Sequences, Nucleic Acid Ribosomes/physiology Viral Proteins/biosynthesis
Chemicals
RNA, Messenger RNA, Viral Viral Proteins Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jang S K
Department of Microbiology, School of Medicine, State University of New York, Stony Brook 11794-8621.
Davies M V
Kaufman R J
Wimmer E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.4.1651-1660.1989
Published
1989-04-00
Pages
1651-60
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248413
Subset
IM
Grants
NIAID NIH HHS · AI-15122 · United States
NCI NIH HHS · CA-28146 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.4.1651-1660.1989 PMC Full Text View journal details
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