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PMID: 8794292 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cellular origin and ultrastructure of membranes induced during poliovirus infection.

Journal of virology ·Vol. 70 ·No. 10 ·1996-10-00 ·Pages 6576-88

Schlegel A, Giddings TH, Ladinsky MS, Kirkegaard K

Abstract

Poliovirus RNA replicative complexes are associated with cytoplasmic membranous structures that accumulate during viral infection. These membranes were immunoisolated by using a monoclonal antibody against the viral nonstructural protein 2C. Biochemical analysis of the isolated membranes revealed that several organelles of the host cell (lysosomes, trans-Golgi stack and trans-Golgi network, and endoplasmic reticulum) contributed to the virus-induced membranous structures. Electron microscopy of infected cells preserved by high-pressure freezing revealed that the virus-induced membranes contain double lipid bilayers that surround apparently cytosolic material. Immunolabeling experiments showed that poliovirus proteins 2C and 3D were localized to the same membranes as the cellular markers tested. The morphological and biochemical data are consistent with the hypothesis that autophagy or a similar host process is involved in the formation of the poliovirus-induced membranes.

MeSH Terms
Cell Membrane/ultrastructure,virology HeLa Cells Humans Microscopy, Electron Poliomyelitis/pathology Poliovirus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schlegel A
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309, USA.
Giddings T H
Ladinsky M S
Kirkegaard K
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.10.6576-6588.1996
Published
1996-10-00
Pages
6576-88
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190698
Subset
IM
Grants
NIAID NIH HHS · AI 25166 · United States
External Links
PubMed source DOI: 10.1128/JVI.70.10.6576-6588.1996 PMC Full Text View journal details
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